Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11332 | 3' | -55.5 | NC_003084.1 | + | 81832 | 0.66 | 0.87903 |
Target: 5'- -------aGCAACAUUCG-GCGCUGCa -3' miRNA: 3'- ccaacggaCGUUGUGAGCuCGCGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 71694 | 0.66 | 0.871632 |
Target: 5'- uGUUGCCagcgaauaUGCAGCucaUCGAcaucuaauucgGCGCUGCc -3' miRNA: 3'- cCAACGG--------ACGUUGug-AGCU-----------CGCGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 54374 | 0.66 | 0.871632 |
Target: 5'- uGGUgcgaCUuuGGCACUUGAGCuGCCGUa -3' miRNA: 3'- -CCAacg-GAcgUUGUGAGCUCG-CGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 61738 | 0.66 | 0.87088 |
Target: 5'- -uUUGCCUGCAuaauuGCACUCcaagugcGAGaCGguCCGCu -3' miRNA: 3'- ccAACGGACGU-----UGUGAG-------CUC-GC--GGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 42838 | 0.66 | 0.864009 |
Target: 5'- ---cGCCaUGUcuCACUCGAGCGa-GCa -3' miRNA: 3'- ccaaCGG-ACGuuGUGAGCUCGCggCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 47020 | 0.66 | 0.864009 |
Target: 5'- cGUUGCagugGCAucgcaagguGCACUUGAGCGgUGUu -3' miRNA: 3'- cCAACGga--CGU---------UGUGAGCUCGCgGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 38318 | 0.66 | 0.864009 |
Target: 5'- ---gGCUUGCAaucgauuccgcaGCAgUCG-GCGUCGCa -3' miRNA: 3'- ccaaCGGACGU------------UGUgAGCuCGCGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 3575 | 0.66 | 0.856166 |
Target: 5'- ----aCCUaGCuGCACUgGuGCGCCGCu -3' miRNA: 3'- ccaacGGA-CGuUGUGAgCuCGCGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 173 | 0.66 | 0.853771 |
Target: 5'- ---gGCCUGCAugGgacuauaauccccgUUCGAGCgggGCUGCa -3' miRNA: 3'- ccaaCGGACGUugU--------------GAGCUCG---CGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 65290 | 0.66 | 0.848111 |
Target: 5'- cGGcgGCUUGCAGCA-UCGGGauguaCCGCa -3' miRNA: 3'- -CCaaCGGACGUUGUgAGCUCgc---GGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 76579 | 0.66 | 0.848111 |
Target: 5'- ---cGuCCaGCAGCGCUCGGGUgaugaaGCUGCu -3' miRNA: 3'- ccaaC-GGaCGUUGUGAGCUCG------CGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 96173 | 0.67 | 0.839849 |
Target: 5'- ---cGCCUGguGCgGCUauucgagagcgaCGAGCGCaCGCu -3' miRNA: 3'- ccaaCGGACguUG-UGA------------GCUCGCG-GCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 62341 | 0.67 | 0.82274 |
Target: 5'- aGGUggGUUUGCGuuuACGCUUGAGCaGCUuGCg -3' miRNA: 3'- -CCAa-CGGACGU---UGUGAGCUCG-CGG-CG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 18505 | 0.67 | 0.82274 |
Target: 5'- aGUUGuUCUGCgAACcCUCGGGCGCUuCa -3' miRNA: 3'- cCAAC-GGACG-UUGuGAGCUCGCGGcG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 73075 | 0.67 | 0.82274 |
Target: 5'- cGUUGCC-GCGA----CGGGCGCUGCu -3' miRNA: 3'- cCAACGGaCGUUgugaGCUCGCGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 105575 | 0.68 | 0.786411 |
Target: 5'- --cUGCUUGUAGCGCUUG-GCGuuGg -3' miRNA: 3'- ccaACGGACGUUGUGAGCuCGCggCg -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 1045 | 0.68 | 0.786411 |
Target: 5'- --cUGCUUGUAGCGCUUG-GCGuuGg -3' miRNA: 3'- ccaACGGACGUUGUGAGCuCGCggCg -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 31959 | 0.68 | 0.776941 |
Target: 5'- aGUUGCCggacucgguugGCAcCACcgCGGGCucgGCCGCa -3' miRNA: 3'- cCAACGGa----------CGUuGUGa-GCUCG---CGGCG- -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 86191 | 0.68 | 0.767335 |
Target: 5'- ---aGUUUGCuACAgUUGAGCGCCGa -3' miRNA: 3'- ccaaCGGACGuUGUgAGCUCGCGGCg -5' |
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11332 | 3' | -55.5 | NC_003084.1 | + | 27683 | 0.68 | 0.757603 |
Target: 5'- cGGgUGCCgaugaUGUAGCGCUC-AGaGCCGCu -3' miRNA: 3'- -CCaACGG-----ACGUUGUGAGcUCgCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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