Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11413 | 5' | -55.4 | NC_003085.1 | + | 43407 | 0.66 | 0.70022 |
Target: 5'- aUCC-CGGUGGgGCUCGgGAGAaucagGUCAu -3' miRNA: 3'- -AGGaGUUACUgCGAGCgCUCUg----CGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 12997 | 0.66 | 0.70022 |
Target: 5'- gCCgcCAGUGGCacuacCUCGCcauGAGGCGCCGc -3' miRNA: 3'- aGGa-GUUACUGc----GAGCG---CUCUGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 30785 | 0.66 | 0.689307 |
Target: 5'- aCCUCGAccgGAagaGC-CGUGAGcGCGCCGc -3' miRNA: 3'- aGGAGUUa--CUg--CGaGCGCUC-UGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 17020 | 0.66 | 0.689307 |
Target: 5'- ----gGAUGGgcgcUGCUCGCGuauGACGCCAa -3' miRNA: 3'- aggagUUACU----GCGAGCGCu--CUGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 32420 | 0.66 | 0.689307 |
Target: 5'- cCCUCGAagcuccggagguUGGCGUcauaCGCGAGcaGCGCCc -3' miRNA: 3'- aGGAGUU------------ACUGCGa---GCGCUC--UGCGGu -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 3506 | 0.66 | 0.678337 |
Target: 5'- -gCUCGccgucUGGCGuCUCGCGAG-CGUCAu -3' miRNA: 3'- agGAGUu----ACUGC-GAGCGCUCuGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 46519 | 0.66 | 0.678337 |
Target: 5'- aCCUCuuUGGC-CUCGUGcucGACGUCAc -3' miRNA: 3'- aGGAGuuACUGcGAGCGCu--CUGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 27448 | 0.66 | 0.678337 |
Target: 5'- cCUUCGAaaugGGCGCgcaguuucgUGCGGGAgGCCAc -3' miRNA: 3'- aGGAGUUa---CUGCGa--------GCGCUCUgCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 5246 | 0.66 | 0.676138 |
Target: 5'- gCCUCcaacgcgaaugGGUGGCGCguggugggugcgCGaCGGGACGCCc -3' miRNA: 3'- aGGAG-----------UUACUGCGa-----------GC-GCUCUGCGGu -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 33023 | 0.66 | 0.671734 |
Target: 5'- gCCUCgAAUGACGUcggCGCcuggauauggggccaGAGACGCUu -3' miRNA: 3'- aGGAG-UUACUGCGa--GCG---------------CUCUGCGGu -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 45029 | 0.66 | 0.667324 |
Target: 5'- cUCgUCAggGAUGCUCuGCcAGuCGCCAg -3' miRNA: 3'- -AGgAGUuaCUGCGAG-CGcUCuGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 40638 | 0.66 | 0.667324 |
Target: 5'- gUCCUCGucgcccUGACGCUgGCu--GCGCCGc -3' miRNA: 3'- -AGGAGUu-----ACUGCGAgCGcucUGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 40906 | 0.66 | 0.667324 |
Target: 5'- aCCUCAagaccugcGUGACgaGCUCGCcgcGCGCCGa -3' miRNA: 3'- aGGAGU--------UACUG--CGAGCGcucUGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 627 | 0.66 | 0.667324 |
Target: 5'- -gCUCGAggcUGGCGCggaccUCGuCGAGGCGCUc -3' miRNA: 3'- agGAGUU---ACUGCG-----AGC-GCUCUGCGGu -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 8336 | 0.66 | 0.656276 |
Target: 5'- uUCCUCAucgGGCGCguggaGCGgcuggaagaaagGGugGCCGg -3' miRNA: 3'- -AGGAGUua-CUGCGag---CGC------------UCugCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 5092 | 0.66 | 0.656276 |
Target: 5'- cCCgagcgaCAGUGACGUUgaCGCGGGAUugGCCGc -3' miRNA: 3'- aGGa-----GUUACUGCGA--GCGCUCUG--CGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 24047 | 0.66 | 0.645206 |
Target: 5'- aUCCUCGcccgccUGGCGaagCGCGGGAaGCCGc -3' miRNA: 3'- -AGGAGUu-----ACUGCga-GCGCUCUgCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 9862 | 0.66 | 0.645206 |
Target: 5'- -gCUCGGUG-CGCUcCGCGAcGCGCUc -3' miRNA: 3'- agGAGUUACuGCGA-GCGCUcUGCGGu -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 47683 | 0.67 | 0.634124 |
Target: 5'- aCUUCGGcggucUGACGCUCagauGCGAGuCGUCGa -3' miRNA: 3'- aGGAGUU-----ACUGCGAG----CGCUCuGCGGU- -5' |
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11413 | 5' | -55.4 | NC_003085.1 | + | 5703 | 0.67 | 0.616393 |
Target: 5'- cCCUCGAUGGCGggacucggccgucuaCUCGCGGGccgagguCGUCGc -3' miRNA: 3'- aGGAGUUACUGC---------------GAGCGCUCu------GCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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