Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11414 | 5' | -51.1 | NC_003085.1 | + | 14077 | 0.7 | 0.674311 |
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Target: 5'- cGCAgagCCCGAUGUgaacgaacucAACUgGAGGUCcACg -3' miRNA: 3'- -CGUa--GGGUUGCG----------UUGAgCUCCAGuUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 33205 | 0.7 | 0.662935 |
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Target: 5'- gGCGUCCUggaggcggcGGCGCAGCaCGAGGaugaCGACg -3' miRNA: 3'- -CGUAGGG---------UUGCGUUGaGCUCCa---GUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 30837 | 0.71 | 0.640102 |
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Target: 5'- gGCAaCCCAGCaGCAACUCGA---CAACg -3' miRNA: 3'- -CGUaGGGUUG-CGUUGAGCUccaGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 18336 | 0.71 | 0.617238 |
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Target: 5'- cGCAcgaaCUCGGCGCGcuGCUCGGGGUUGAg -3' miRNA: 3'- -CGUa---GGGUUGCGU--UGAGCUCCAGUUg -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 48810 | 0.73 | 0.516158 |
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Target: 5'- aCGUCCgGGCGguGCUCGAGcGccUCGACg -3' miRNA: 3'- cGUAGGgUUGCguUGAGCUC-C--AGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 3093 | 0.73 | 0.494517 |
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Target: 5'- cGC-UCCCGcggaagGCGCGGCUUGAGG-CGAUg -3' miRNA: 3'- -CGuAGGGU------UGCGUUGAGCUCCaGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 43404 | 0.74 | 0.452547 |
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Target: 5'- uGCAUCCCggUGgGGCUCGggagaaucAGGUCAu- -3' miRNA: 3'- -CGUAGGGuuGCgUUGAGC--------UCCAGUug -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 6935 | 0.79 | 0.229178 |
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Target: 5'- cGCAUCCCGG-GCGGCUgGGcGGUCGACu -3' miRNA: 3'- -CGUAGGGUUgCGUUGAgCU-CCAGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 23458 | 0.79 | 0.229178 |
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Target: 5'- cGCAUgacggaggCCCAGCGCAGCgUCGAGGcauucgUCAGCa -3' miRNA: 3'- -CGUA--------GGGUUGCGUUG-AGCUCC------AGUUG- -5' |
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| 11414 | 5' | -51.1 | NC_003085.1 | + | 45783 | 1.12 | 0.001494 |
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Target: 5'- cGCAUCCCAACGCAACUCGAGGUCAACc -3' miRNA: 3'- -CGUAGGGUUGCGUUGAGCUCCAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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