Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11421 | 5' | -57.1 | NC_003085.1 | + | 36727 | 0.66 | 0.572083 |
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Target: 5'- cGCaccacCUCCGGCC-CCUUUUCUUgCCg -3' miRNA: 3'- cCGacc--GAGGCCGGuGGGAAAGAA-GG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 2310 | 0.66 | 0.572083 |
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Target: 5'- cGCUGGCcugccucgccaCCGGCCGCCg---CUUCa -3' miRNA: 3'- cCGACCGa----------GGCCGGUGGgaaaGAAGg -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 24142 | 0.66 | 0.572083 |
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Target: 5'- cGGCUGGUacUCCGGCUAUgaCgugCUaCCg -3' miRNA: 3'- -CCGACCG--AGGCCGGUGg-GaaaGAaGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 27179 | 0.66 | 0.572083 |
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Target: 5'- cGCUGGCUUCGGCguCUCUcacgcaUUCgUCa -3' miRNA: 3'- cCGACCGAGGCCGguGGGA------AAGaAGg -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 8623 | 0.66 | 0.582905 |
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Target: 5'- aGGUUGGCcagcCCGGCCGCCg--UCa--- -3' miRNA: 3'- -CCGACCGa---GGCCGGUGGgaaAGaagg -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 34429 | 0.66 | 0.582905 |
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Target: 5'- gGGCUgcGGCUUgGGCCgGCCCag-CggcaagUCCa -3' miRNA: 3'- -CCGA--CCGAGgCCGG-UGGGaaaGa-----AGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 36029 | 0.66 | 0.582905 |
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Target: 5'- cGGCcaugGGCacgCCGuaUGCCCccgUCUUCCg -3' miRNA: 3'- -CCGa---CCGa--GGCcgGUGGGaa-AGAAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 28329 | 0.66 | 0.604661 |
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Target: 5'- -----cUUCCGGCCGCCCUUg--UCCc -3' miRNA: 3'- ccgaccGAGGCCGGUGGGAAagaAGG- -5' |
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| 11421 | 5' | -57.1 | NC_003085.1 | + | 13558 | 0.66 | 0.615579 |
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Target: 5'- cGGCUGGCcgUGGCaugGCCCUgggacUCCg -3' miRNA: 3'- -CCGACCGagGCCGg--UGGGAaaga-AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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