Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11425 | 5' | -58.1 | NC_003085.1 | + | 5967 | 0.7 | 0.299801 |
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Target: 5'- cCGGGCUGcUGGAGGc--GGUGGGCCGu -3' miRNA: 3'- -GUCCGGCuACUUCUucuCCGCCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 5462 | 0.66 | 0.480711 |
|
Target: 5'- gAGGcCCGAgugcgGGAGuuGGAGGCggacaacgcggcgugGGGCCGg -3' miRNA: 3'- gUCC-GGCUa----CUUCu-UCUCCG---------------CCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 5017 | 0.74 | 0.16826 |
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Target: 5'- gCAGGCCGA-GAAGAcgacgacgcccgaGGAGGUGGcGCUg -3' miRNA: 3'- -GUCCGGCUaCUUCU-------------UCUCCGCC-CGGu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 4483 | 0.68 | 0.39947 |
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Target: 5'- aGGGCaCGGUGAcGAAGcgcgcuGGCgacuGGGCCAa -3' miRNA: 3'- gUCCG-GCUACUuCUUCu-----CCG----CCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 1247 | 0.71 | 0.238462 |
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Target: 5'- gGGGCUGcgGAGGcAGGuggccgaauccaAGGUGGGCCAc -3' miRNA: 3'- gUCCGGCuaCUUC-UUC------------UCCGCCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 487 | 0.66 | 0.528466 |
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Target: 5'- -cGGCCaucagcgcggaGAUGAAGgcGc-GCGGGCCGg -3' miRNA: 3'- guCCGG-----------CUACUUCuuCucCGCCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 118 | 0.66 | 0.497097 |
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Target: 5'- gCGGGCCcccaauGUGggGAGaGGGCGGGaCGg -3' miRNA: 3'- -GUCCGGc-----UACuuCUUcUCCGCCCgGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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