Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11425 | 5' | -58.1 | NC_003085.1 | + | 18876 | 0.7 | 0.314988 |
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Target: 5'- aAGGCUGAUGGAGccG-GGCGaGGuCCAc -3' miRNA: 3'- gUCCGGCUACUUCuuCuCCGC-CC-GGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 40849 | 0.68 | 0.380568 |
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Target: 5'- cCGGGCUGGccaaccUGGAGGccagcguggacaaGGAGGUGGGCa- -3' miRNA: 3'- -GUCCGGCU------ACUUCU-------------UCUCCGCCCGgu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 11956 | 0.68 | 0.381454 |
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Target: 5'- uGGGC--GUGAAGGAGGGGCGGcGUgAg -3' miRNA: 3'- gUCCGgcUACUUCUUCUCCGCC-CGgU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 7261 | 0.68 | 0.390394 |
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Target: 5'- aCAGGCUcugGGUGAAGGAGAcguGGCGcaccGCCGc -3' miRNA: 3'- -GUCCGG---CUACUUCUUCU---CCGCc---CGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 43827 | 0.67 | 0.446803 |
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Target: 5'- --aGCCGGUGuuGAGGucgcgcAGGCGGGCg- -3' miRNA: 3'- gucCGGCUACuuCUUC------UCCGCCCGgu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 32980 | 0.66 | 0.507465 |
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Target: 5'- gCAGGCCGGguuugGAAGGAGAGa-GGuCCAu -3' miRNA: 3'- -GUCCGGCUa----CUUCUUCUCcgCCcGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 18534 | 0.71 | 0.257654 |
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Target: 5'- gCGGGCCGAUugcccaGAAgcacGAGGuGGCGGGCa- -3' miRNA: 3'- -GUCCGGCUA------CUU----CUUCuCCGCCCGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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