Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11425 | 5' | -58.1 | NC_003085.1 | + | 47152 | 0.71 | 0.257654 |
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Target: 5'- -cGGCCGGUGGc---GAGGCaGGCCAg -3' miRNA: 3'- guCCGGCUACUucuuCUCCGcCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 18534 | 0.71 | 0.257654 |
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Target: 5'- gCGGGCCGAUugcccaGAAgcacGAGGuGGCGGGCa- -3' miRNA: 3'- -GUCCGGCUA------CUU----CUUCuCCGCCCGgu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 1247 | 0.71 | 0.238462 |
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Target: 5'- gGGGCUGcgGAGGcAGGuggccgaauccaAGGUGGGCCAc -3' miRNA: 3'- gUCCGGCuaCUUC-UUC------------UCCGCCCGGU- -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 25755 | 0.74 | 0.168718 |
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Target: 5'- gGGGCCGucuUGAcGGAGGAGGCugcugcgcGGGCCc -3' miRNA: 3'- gUCCGGCu--ACU-UCUUCUCCG--------CCCGGu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 5017 | 0.74 | 0.16826 |
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Target: 5'- gCAGGCCGA-GAAGAcgacgacgcccgaGGAGGUGGcGCUg -3' miRNA: 3'- -GUCCGGCUaCUUCU-------------UCUCCGCC-CGGu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 40484 | 0.76 | 0.121153 |
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Target: 5'- cCGGGCCGGUGAAGuccgcGAGGCcGcGGCCc -3' miRNA: 3'- -GUCCGGCUACUUCuu---CUCCG-C-CCGGu -5' |
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| 11425 | 5' | -58.1 | NC_003085.1 | + | 40124 | 1.08 | 0.000471 |
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Target: 5'- aCAGGCCGAUGAAGAAGAGGCGGGCCAu -3' miRNA: 3'- -GUCCGGCUACUUCUUCUCCGCCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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