Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11438 | 3' | -58.4 | NC_003085.1 | + | 16381 | 0.71 | 0.282339 |
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Target: 5'- uGAGCCUc-CGCGgGCaCGUCCugGCGa -3' miRNA: 3'- gCUCGGAcaGUGUgCG-GCAGGugCGU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 15192 | 0.67 | 0.431079 |
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Target: 5'- uGAGCCgGUgCGCcaGCGCCGcgCCGCGgAa -3' miRNA: 3'- gCUCGGaCA-GUG--UGCGGCa-GGUGCgU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 14735 | 0.67 | 0.449269 |
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Target: 5'- gCGAuGCCaucgguaUGcCACGCGCCGccguggCCAUGCAa -3' miRNA: 3'- -GCU-CGG-------ACaGUGUGCGGCa-----GGUGCGU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 12932 | 0.66 | 0.520686 |
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Target: 5'- gCGGG-CUGUCAguCGCCGUCgcuaUACGUg -3' miRNA: 3'- -GCUCgGACAGUguGCGGCAG----GUGCGu -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 12376 | 0.67 | 0.440599 |
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Target: 5'- gGAGCgacu-GCGCGCCGUCCACcuGCAu -3' miRNA: 3'- gCUCGgacagUGUGCGGCAGGUG--CGU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 5558 | 0.69 | 0.376602 |
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Target: 5'- aGGGCCUGgCGCcgcgaacgguGCGCCacGUCUACGCc -3' miRNA: 3'- gCUCGGACaGUG----------UGCGG--CAGGUGCGu -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 4671 | 0.68 | 0.403259 |
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Target: 5'- gCGAGCCUuUCACgGCGCCGagUUGCGCc -3' miRNA: 3'- -GCUCGGAcAGUG-UGCGGCa-GGUGCGu -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 1178 | 0.68 | 0.394241 |
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Target: 5'- gGAGCC---CACGCGCUGcUCUGCGCGu -3' miRNA: 3'- gCUCGGacaGUGUGCGGC-AGGUGCGU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 1018 | 0.7 | 0.311529 |
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Target: 5'- -uGGCCUaugcUCGCGgccuCGCCGUCCAUGCGg -3' miRNA: 3'- gcUCGGAc---AGUGU----GCGGCAGGUGCGU- -5' |
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| 11438 | 3' | -58.4 | NC_003085.1 | + | 581 | 0.7 | 0.319175 |
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Target: 5'- gCGAGCCcccgccgcagggUGUcucgaCugGCGCCGUCCuggGCGCGc -3' miRNA: 3'- -GCUCGG------------ACA-----GugUGCGGCAGG---UGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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