Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11483 | 5' | -56.1 | NC_003085.1 | + | 21880 | 0.7 | 0.350233 |
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Target: 5'- --gUGGCcgaacUGGCCGGGuccGUgCCC-UGCCa -3' miRNA: 3'- aaaACCG-----ACCGGCCU---UAgGGGuACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 16235 | 0.71 | 0.333454 |
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Target: 5'- ---cGGCUGGCguCGucAUCCUCGUGCUg -3' miRNA: 3'- aaaaCCGACCG--GCcuUAGGGGUACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 48568 | 0.71 | 0.317271 |
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Target: 5'- ---gGGUUGGCaCGGGAUgCCCCucggacGCCa -3' miRNA: 3'- aaaaCCGACCG-GCCUUA-GGGGua----CGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 15142 | 0.71 | 0.317271 |
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Target: 5'- --cUGGCUGaaGCUGGAcgCCUCgGUGCCc -3' miRNA: 3'- aaaACCGAC--CGGCCUuaGGGG-UACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 38822 | 0.71 | 0.317271 |
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Target: 5'- cUUUGGCUGccucuGCCGGcg-CCCCAcacaGCCg -3' miRNA: 3'- aAAACCGAC-----CGGCCuuaGGGGUa---CGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 7712 | 0.73 | 0.2199 |
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Target: 5'- ---aGGCUGGCCGGGcGUCCgagcaggaaaacuCgGUGCCg -3' miRNA: 3'- aaaaCCGACCGGCCU-UAGG-------------GgUACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 1803 | 0.78 | 0.112956 |
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Target: 5'- -gUUGGCUGGCCGGGAcuggugcgCCCCcUGUUg -3' miRNA: 3'- aaAACCGACCGGCCUUa-------GGGGuACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 3061 | 0.82 | 0.054409 |
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Target: 5'- gUUUGGCccgccGCCGGAAUCCCC-UGCCa -3' miRNA: 3'- aAAACCGac---CGGCCUUAGGGGuACGG- -5' |
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| 11483 | 5' | -56.1 | NC_003085.1 | + | 15046 | 1.1 | 0.000482 |
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Target: 5'- gUUUUGGCUGGCCGGAAUCCCCAUGCCg -3' miRNA: 3'- -AAAACCGACCGGCCUUAGGGGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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