Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11572 | 3' | -61 | NC_003085.1 | + | 30044 | 0.72 | 0.156557 |
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Target: 5'- cGGUGGAUGaCUGcUUGCcggaGGGCUGCUGg -3' miRNA: 3'- -UCGCCUAC-GGC-AACGag--CCCGGCGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 10734 | 0.72 | 0.148356 |
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Target: 5'- aAGCGGAcGCUGUUGCUguccggccacaCGGGCgccgggaagaCGCUGg -3' miRNA: 3'- -UCGCCUaCGGCAACGA-----------GCCCG----------GCGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 34620 | 0.74 | 0.103969 |
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Target: 5'- cGCGGGccgGCUGguggcGCUCGcGGCCGCUGu -3' miRNA: 3'- uCGCCUa--CGGCaa---CGAGC-CCGGCGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 44943 | 0.75 | 0.093039 |
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Target: 5'- uGCGGAUGCgGUUGCgaGGGCC-CUGc -3' miRNA: 3'- uCGCCUACGgCAACGagCCCGGcGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 12393 | 0.77 | 0.059318 |
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Target: 5'- gGGcCGGGUGCUGcUGCUCgacccggcgGGGCCGCUGc -3' miRNA: 3'- -UC-GCCUACGGCaACGAG---------CCCGGCGAC- -5' |
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| 11572 | 3' | -61 | NC_003085.1 | + | 26372 | 1.08 | 0.000265 |
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Target: 5'- cAGCGGAUGCCGUUGCUCGGGCCGCUGa -3' miRNA: 3'- -UCGCCUACGGCAACGAGCCCGGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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