Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11595 | 3' | -55.7 | NC_003085.1 | + | 12010 | 0.69 | 0.476381 |
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Target: 5'- aGcGGCCUGACgcugGCGCAccugccGGUgCUGCGCg -3' miRNA: 3'- gC-CCGGACUGa---CGUGUa-----UCA-GGUGCG- -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 19415 | 0.7 | 0.436521 |
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Target: 5'- uGGGCCUG-CUGCAacuuGUgCugGCc -3' miRNA: 3'- gCCCGGACuGACGUguauCAgGugCG- -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 38339 | 0.7 | 0.39865 |
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Target: 5'- uCGGGCCUGGCacGCGCAacuUCCugGg -3' miRNA: 3'- -GCCCGGACUGa-CGUGUaucAGGugCg -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 47434 | 0.72 | 0.313692 |
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Target: 5'- gCGGGCCaaacugcuccaUGucCUGCGCGUcgaagucgaaccGGUCCGCGUa -3' miRNA: 3'- -GCCCGG-----------ACu-GACGUGUA------------UCAGGUGCG- -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 5558 | 0.75 | 0.196688 |
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Target: 5'- aGGGCCUGGCgccgcgaacggUGCGCcacGUCUACGCc -3' miRNA: 3'- gCCCGGACUG-----------ACGUGuauCAGGUGCG- -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 18098 | 0.76 | 0.173622 |
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Target: 5'- -uGGCCUGgaagcgGCUGCGCAUGgcggccuaccgguacGUCCGCGCg -3' miRNA: 3'- gcCCGGAC------UGACGUGUAU---------------CAGGUGCG- -5' |
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| 11595 | 3' | -55.7 | NC_003085.1 | + | 36699 | 1.11 | 0.000566 |
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Target: 5'- cCGGGCCUGACUGCACAUAGUCCACGCc -3' miRNA: 3'- -GCCCGGACUGACGUGUAUCAGGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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