Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11600 | 5' | -57.6 | NC_003085.1 | + | 37902 | 0.74 | 0.167739 |
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Target: 5'- -gGGCAGGACGgugCGUUGGCGCaGGCc- -3' miRNA: 3'- caCCGUCUUGCa--GCAGUCGCG-CCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 45402 | 0.74 | 0.186411 |
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Target: 5'- -aGGCGGAGCGcCGUCagcgaaagcauggGGCGCGGUg- -3' miRNA: 3'- caCCGUCUUGCaGCAG-------------UCGCGCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 28181 | 0.69 | 0.353833 |
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Target: 5'- -gGGcCAGGACGaaGUCugGGCGCGGCa- -3' miRNA: 3'- caCC-GUCUUGCagCAG--UCGCGCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 5999 | 0.69 | 0.370976 |
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Target: 5'- aGUGGguGcguGACGUCaucgcucagCAGCGCGGCa- -3' miRNA: 3'- -CACCguC---UUGCAGca-------GUCGCGCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 11567 | 0.69 | 0.370976 |
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Target: 5'- -gGGCA--GCGcCGUCGGCGUGGUg- -3' miRNA: 3'- caCCGUcuUGCaGCAGUCGCGCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 39876 | 0.66 | 0.526553 |
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Target: 5'- aUGGCGGAgagACGcCGgugcUCGGCGgCGGCa- -3' miRNA: 3'- cACCGUCU---UGCaGC----AGUCGC-GCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 18513 | 0.66 | 0.537169 |
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Target: 5'- cUGGCAGGAC-UCGggucUGCGGCUGc -3' miRNA: 3'- cACCGUCUUGcAGCagucGCGCCGAC- -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 20602 | 0.66 | 0.546785 |
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Target: 5'- -cGGCAGcGCcgcggaaGUCGggCGGCGCGGgUGc -3' miRNA: 3'- caCCGUCuUG-------CAGCa-GUCGCGCCgAC- -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 34316 | 0.66 | 0.56293 |
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Target: 5'- -gGGCAGuaguGCGucUCGUgcucgucgcaccacgCGGCGCGGCg- -3' miRNA: 3'- caCCGUCu---UGC--AGCA---------------GUCGCGCCGac -5' |
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| 11600 | 5' | -57.6 | NC_003085.1 | + | 26709 | 0.84 | 0.035918 |
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Target: 5'- -cGGCGG-GCGUCGUCGGCGCGGgUGu -3' miRNA: 3'- caCCGUCuUGCAGCAGUCGCGCCgAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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