miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1163 3' -48.9 NC_001132.2 + 153891 0.66 0.999085
Target:  5'- aCGAGGGUgauGUCGCUCaugg---UACCg -3'
miRNA:   3'- aGUUCCCG---CAGCGAGcaguuuaAUGG- -5'
1163 3' -48.9 NC_001132.2 + 6855 0.68 0.996703
Target:  5'- -aAGGGGCGUCuGUacugCGUCGGAggACg -3'
miRNA:   3'- agUUCCCGCAG-CGa---GCAGUUUaaUGg -5'
1163 3' -48.9 NC_001132.2 + 160621 0.68 0.99612
Target:  5'- cCAAGGaGCGUCGCUggacaGUCGGAc---- -3'
miRNA:   3'- aGUUCC-CGCAGCGAg----CAGUUUaaugg -5'
1163 3' -48.9 NC_001132.2 + 59262 0.68 0.9947
Target:  5'- aUCGAGGGgGUCG-UCGUUuaacuugGCCc -3'
miRNA:   3'- -AGUUCCCgCAGCgAGCAGuuuaa--UGG- -5'
1163 3' -48.9 NC_001132.2 + 46119 1 0.059295
Target:  5'- gUCAAGGGCGUCGUcCGUCAAAUUACCc -3'
miRNA:   3'- -AGUUCCCGCAGCGaGCAGUUUAAUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.