Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11653 | 3' | -55.8 | NC_003102.1 | + | 114496 | 0.66 | 0.918989 |
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Target: 5'- aCGC-UUGAGCGUCGaucGUCuuugauCGCGCa -3' miRNA: 3'- aGCGcAGCUCGCGGUa--CAGuu----GCGCG- -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 117927 | 0.68 | 0.826421 |
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Target: 5'- aUGU-UCGAGU-CCAUGUCGcuaACGCGCa -3' miRNA: 3'- aGCGcAGCUCGcGGUACAGU---UGCGCG- -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 121128 | 0.67 | 0.887761 |
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Target: 5'- uUCGUGcCGGucGCGCCGUGUCcgcCGgGUu -3' miRNA: 3'- -AGCGCaGCU--CGCGGUACAGuu-GCgCG- -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 127179 | 0.71 | 0.664379 |
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Target: 5'- uUCGCGgccacguccauUCGaAGCGCCA-GUCuGCGCGg -3' miRNA: 3'- -AGCGC-----------AGC-UCGCGGUaCAGuUGCGCg -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 128583 | 0.71 | 0.643972 |
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Target: 5'- gCGCGUUGAGCGCgCuagacaGACGCGUc -3' miRNA: 3'- aGCGCAGCUCGCG-Guacag-UUGCGCG- -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 128748 | 0.69 | 0.763438 |
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Target: 5'- aCGCGUCugucuAGCGCgc--UCAACGCGCu -3' miRNA: 3'- aGCGCAGc----UCGCGguacAGUUGCGCG- -5' |
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| 11653 | 3' | -55.8 | NC_003102.1 | + | 133740 | 0.69 | 0.753905 |
|
Target: 5'- cCGCGUCGcAGcCGCCguaGUGUCGcCGCa- -3' miRNA: 3'- aGCGCAGC-UC-GCGG---UACAGUuGCGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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