miRNA: 3'- uacuuC-GCAGaGCUGCCGuAGCUAGg -5'
miRNA: 3'- uaCU-UCGCa----GAGCuGCCGUAGCUaGG- -5'
miRNA: 3'- uACU-------UCGCagaGCUGCCGUAGcUAGG- -5'
miRNA: 3'- uaCUUCGCaga-GCUGCCG--------UAGCUAGg -5'
miRNA: 3'- uaCUuCGCAGa----GCUGCCG------UAGCU-AGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11675 | 3' | -52.7 | NC_003102.1 | + | 49546 | 0.66 | 0.97083 |
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Target: 5'- ----cGcCGUCgCGACGGCuUCGAUCg -3' miRNA: 3'- uacuuC-GCAGaGCUGCCGuAGCUAGg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 18469 | 0.66 | 0.973667 |
|
Target: 5'- -cGAcGGCGgcaggUUCGuCGGCGUCGG-CCa -3' miRNA: 3'- uaCU-UCGCa----GAGCuGCCGUAGCUaGG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 62036 | 0.66 | 0.978497 |
|
Target: 5'- uUGAucacuacGGCGa--CGACGGCAUC-AUCCg -3' miRNA: 3'- uACU-------UCGCagaGCUGCCGUAGcUAGG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 71637 | 0.66 | 0.978731 |
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Target: 5'- -aGAGGCGgcaaCGGCGGUgacguugaAUCGAUCg -3' miRNA: 3'- uaCUUCGCaga-GCUGCCG--------UAGCUAGg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 81380 | 0.66 | 0.978731 |
|
Target: 5'- -cGAcGCGUCgucugCGACGGCgaccgcGUCGAcUUCg -3' miRNA: 3'- uaCUuCGCAGa----GCUGCCG------UAGCU-AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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