miRNA: 3'- uaCUUCGCaga-GCUGCCGUAGcUAGg -5'
miRNA: 3'- -UACuuCGCAGAgCugCCg---UAGCUAGg -5'
miRNA: 3'- uACU--UCGCagaGCUGCCGUAGCUAgg -5'
miRNA: 3'- uaCUuCGC--------AGAGCUGCCGUa--GCUAGG- -5'
miRNA: 3'- -UACUUCGCAGAGCUGCCGUAGCUAGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11675 | 3' | -52.7 | NC_003102.1 | + | 57049 | 0.71 | 0.831706 |
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Target: 5'- -gGAGGCGgaggCGACGGCAUC-AUCa -3' miRNA: 3'- uaCUUCGCaga-GCUGCCGUAGcUAGg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 83103 | 0.71 | 0.823054 |
|
Target: 5'- aAUGggGCGUUUgGugGGacaaGUUGAUCg -3' miRNA: 3'- -UACuuCGCAGAgCugCCg---UAGCUAGg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 49327 | 0.71 | 0.805211 |
|
Target: 5'- uUGAgcAGCGg--CGGCGGCAUCGGUa- -3' miRNA: 3'- uACU--UCGCagaGCUGCCGUAGCUAgg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 63066 | 0.74 | 0.686992 |
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Target: 5'- -cGAcGCGagacacgaUCUCGGCGGCGgcaCGGUCCa -3' miRNA: 3'- uaCUuCGC--------AGAGCUGCCGUa--GCUAGG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 99544 | 1.09 | 0.005268 |
|
Target: 5'- aAUGAAGCGUCUCGACGGCAUCGAUCCc -3' miRNA: 3'- -UACUUCGCAGAGCUGCCGUAGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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