Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11675 | 3' | -52.7 | NC_003102.1 | + | 135518 | 0.67 | 0.952919 |
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Target: 5'- -gGAaucGGCGUCgggguuuuugCGAUGGCGUCGuugcgcuAUCCu -3' miRNA: 3'- uaCU---UCGCAGa---------GCUGCCGUAGC-------UAGG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 75749 | 0.67 | 0.949127 |
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Target: 5'- gAUGAuGGUGUCggGACGGaCAUCGAaaCCg -3' miRNA: 3'- -UACU-UCGCAGagCUGCC-GUAGCUa-GG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 37392 | 0.68 | 0.927704 |
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Target: 5'- uUGAAGCGUCUUGgacauuaGCGcagaaacaugaucaGCGUCGAcgaUCCg -3' miRNA: 3'- uACUUCGCAGAGC-------UGC--------------CGUAGCU---AGG- -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 116696 | 0.68 | 0.918711 |
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Target: 5'- -cGAAGCcg--UGACGGUGUCGAUCa -3' miRNA: 3'- uaCUUCGcagaGCUGCCGUAGCUAGg -5' |
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| 11675 | 3' | -52.7 | NC_003102.1 | + | 131643 | 0.69 | 0.88651 |
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Target: 5'- -cGAGGCGUgUgCGACuGUggCGAUCCg -3' miRNA: 3'- uaCUUCGCAgA-GCUGcCGuaGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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