Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11850 | 3' | -51 | NC_003266.2 | + | 6296 | 0.68 | 0.702894 |
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Target: 5'- gGgGCUGGCAGGucaGAAucGuGCCAGACg -3' miRNA: 3'- gCgCGGCUGUUC---CUUuuCuUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 7048 | 0.69 | 0.678624 |
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Target: 5'- cCGUGCCGACcAGGAcggguucccccuuGAAGAguACUGGAa -3' miRNA: 3'- -GCGCGGCUGuUCCU-------------UUUCU--UGGUCUg -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 8782 | 0.71 | 0.507606 |
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Target: 5'- gCGCGCCGGCcgcggagaccuucagGAGGAGGAGAucgagcaguucaugACCgAGAUu -3' miRNA: 3'- -GCGCGGCUG---------------UUCCUUUUCU--------------UGG-UCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 5293 | 0.72 | 0.462992 |
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Target: 5'- gGCGCCGAUcGGGAGcGGuucucgaccAGCCGGAUg -3' miRNA: 3'- gCGCGGCUGuUCCUUuUC---------UUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 17777 | 0.75 | 0.345737 |
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Target: 5'- aCGgaGCCGGCGGGGGGAaccGGGuCCAGGCg -3' miRNA: 3'- -GCg-CGGCUGUUCCUUU---UCUuGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 9813 | 0.76 | 0.280226 |
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Target: 5'- cCGCGCgGGCGAGGAGGAGGugCccguGGAg -3' miRNA: 3'- -GCGCGgCUGUUCCUUUUCUugG----UCUg -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 6193 | 1.1 | 0.001462 |
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Target: 5'- gCGCGCCGACAAGGAAAAGAACCAGACc -3' miRNA: 3'- -GCGCGGCUGUUCCUUUUCUUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 22466 | 0.66 | 0.831136 |
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Target: 5'- gCGCGCUa--AAGGAGAAccccGACCGGGCc -3' miRNA: 3'- -GCGCGGcugUUCCUUUUc---UUGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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