Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11850 | 3' | -51 | NC_003266.2 | + | 20347 | 0.68 | 0.702894 |
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Target: 5'- gGUGCCGuCGAGGuaggggauGGAGCCcgaguAGACg -3' miRNA: 3'- gCGCGGCuGUUCCuuu-----UCUUGG-----UCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 22466 | 0.66 | 0.831136 |
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Target: 5'- gCGCGCUa--AAGGAGAAccccGACCGGGCc -3' miRNA: 3'- -GCGCGGcugUUCCUUUUc---UUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 23991 | 0.67 | 0.769902 |
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Target: 5'- uCGgGCCGcaaacgcucuGCAAGGAGAAGGAggAGAUc -3' miRNA: 3'- -GCgCGGC----------UGUUCCUUUUCUUggUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 25453 | 0.68 | 0.741402 |
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Target: 5'- cCGC-CCGugGAGGAuuuggaggaagacugGGAGAGCaguCAGGCa -3' miRNA: 3'- -GCGcGGCugUUCCU---------------UUUCUUG---GUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 25968 | 0.67 | 0.769902 |
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Target: 5'- gGCGgCGGCAGGuGGAcuGAGGAUCGcGGCg -3' miRNA: 3'- gCGCgGCUGUUC-CUU--UUCUUGGU-CUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 26000 | 0.72 | 0.451373 |
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Target: 5'- aCGaGCCGGCGcagacccGGGAacuGAGGAACCGGAUc -3' miRNA: 3'- -GCgCGGCUGU-------UCCU---UUUCUUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 31088 | 0.66 | 0.821452 |
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Target: 5'- uGCuGCUGGCGAaugcGGAGuuGGGCgAGACg -3' miRNA: 3'- gCG-CGGCUGUU----CCUUuuCUUGgUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 33141 | 0.68 | 0.736936 |
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Target: 5'- uCGgGUCGGuCAAGGAGAuGAAacccuCCGGGCa -3' miRNA: 3'- -GCgCGGCU-GUUCCUUUuCUU-----GGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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