Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11850 | 3' | -51 | NC_003266.2 | + | 17074 | 0.67 | 0.791098 |
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Target: 5'- gCGCGCCcgcGGCAGGGucAGAGGcgcgcgGCCGcGGCg -3' miRNA: 3'- -GCGCGG---CUGUUCCu-UUUCU------UGGU-CUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 5069 | 0.66 | 0.811543 |
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Target: 5'- uGgGgCGACugcGGGAGuAGGgcGCCAGACg -3' miRNA: 3'- gCgCgGCUGu--UCCUUuUCU--UGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 595 | 0.66 | 0.815533 |
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Target: 5'- uGC-CCGAUGAGGAAAuuaucaucgcuuccgGGAGCgAGAUu -3' miRNA: 3'- gCGcGGCUGUUCCUUU---------------UCUUGgUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 31088 | 0.66 | 0.821452 |
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Target: 5'- uGCuGCUGGCGAaugcGGAGuuGGGCgAGACg -3' miRNA: 3'- gCG-CGGCUGUU----CCUUuuCUUGgUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 11122 | 0.66 | 0.821452 |
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Target: 5'- gGUGCUgaggGACGAGGAuuucGAGgCGGACg -3' miRNA: 3'- gCGCGG----CUGUUCCUuuu-CUUgGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 22466 | 0.66 | 0.831136 |
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Target: 5'- gCGCGCUa--AAGGAGAAccccGACCGGGCc -3' miRNA: 3'- -GCGCGGcugUUCCUUUUc---UUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 17803 | 0.66 | 0.840582 |
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Target: 5'- cCGUcCCGACGcGGAGGAGAcgcugcugacgcACaCGGACg -3' miRNA: 3'- -GCGcGGCUGUuCCUUUUCU------------UG-GUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 3361 | 0.66 | 0.840582 |
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Target: 5'- aGUGUuuUGGgGAGGGAGAGGACCugcauaagGGGCa -3' miRNA: 3'- gCGCG--GCUgUUCCUUUUCUUGG--------UCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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