Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11850 | 3' | -51 | NC_003266.2 | + | 11783 | 0.72 | 0.462992 |
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Target: 5'- gGgGCCGGgacCGAGGggGAGAGCUAcuuuGACa -3' miRNA: 3'- gCgCGGCU---GUUCCuuUUCUUGGU----CUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 5293 | 0.72 | 0.462992 |
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Target: 5'- gGCGCCGAUcGGGAGcGGuucucgaccAGCCGGAUg -3' miRNA: 3'- gCGCGGCUGuUCCUUuUC---------UUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 26000 | 0.72 | 0.451373 |
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Target: 5'- aCGaGCCGGCGcagacccGGGAacuGAGGAACCGGAUc -3' miRNA: 3'- -GCgCGGCUGU-------UCCU---UUUCUUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 15843 | 0.73 | 0.42153 |
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Target: 5'- aGCGCCGGC-AGGAcucGGAGAcgcgcgGCCAcGGCg -3' miRNA: 3'- gCGCGGCUGuUCCU---UUUCU------UGGU-CUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 8401 | 0.73 | 0.42153 |
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Target: 5'- aGCGgCGGCGAGGAcgcGcGCCGGGCg -3' miRNA: 3'- gCGCgGCUGUUCCUuuuCuUGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 17777 | 0.75 | 0.345737 |
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Target: 5'- aCGgaGCCGGCGGGGGGAaccGGGuCCAGGCg -3' miRNA: 3'- -GCg-CGGCUGUUCCUUU---UCUuGGUCUG- -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 9813 | 0.76 | 0.280226 |
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Target: 5'- cCGCGCgGGCGAGGAGGAGGugCccguGGAg -3' miRNA: 3'- -GCGCGgCUGUUCCUUUUCUugG----UCUg -5' |
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| 11850 | 3' | -51 | NC_003266.2 | + | 6193 | 1.1 | 0.001462 |
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Target: 5'- gCGCGCCGACAAGGAAAAGAACCAGACc -3' miRNA: 3'- -GCGCGGCUGUUCCUUUUCUUGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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