Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11894 | 3' | -53.9 | NC_003266.2 | + | 17338 | 0.66 | 0.645839 |
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Target: 5'- -gCCUgc-ACCGCCACggaaGCAAUGcCGGg -3' miRNA: 3'- agGGAaguUGGUGGUGg---CGUUAC-GCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 18386 | 0.7 | 0.437654 |
|
Target: 5'- aCCCacgaugUGACCACCgACCGCAGccaGCGGc -3' miRNA: 3'- aGGGaa----GUUGGUGG-UGGCGUUa--CGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 19778 | 1.12 | 0.000487 |
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Target: 5'- aUCCCUUCAACCACCACCGCAAUGCGGg -3' miRNA: 3'- -AGGGAAGUUGGUGGUGGCGUUACGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 19964 | 0.7 | 0.458003 |
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Target: 5'- uUCCCUuggCAACgACCugCGCAcaGaCGGg -3' miRNA: 3'- -AGGGAa--GUUGgUGGugGCGUuaC-GCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 20102 | 0.69 | 0.521852 |
|
Target: 5'- aUCCUUCAACgACUACCuCucgGCGGc -3' miRNA: 3'- aGGGAAGUUGgUGGUGGcGuuaCGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 22991 | 0.66 | 0.691237 |
|
Target: 5'- --gCUUCAGCUACCcCC-CcGUGCGGa -3' miRNA: 3'- aggGAAGUUGGUGGuGGcGuUACGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 23130 | 0.67 | 0.588863 |
|
Target: 5'- cCCCUcgccUCAggaacaaccACCGCCGCCGCA--GCa- -3' miRNA: 3'- aGGGA----AGU---------UGGUGGUGGCGUuaCGcc -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 24850 | 0.67 | 0.634427 |
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Target: 5'- aUCCUgcCGGCCACCugcuCCGCGcUGCc- -3' miRNA: 3'- -AGGGaaGUUGGUGGu---GGCGUuACGcc -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 26019 | 0.72 | 0.352978 |
|
Target: 5'- gUCCaCUgCcGCCGCCGCCGC--UGUGGa -3' miRNA: 3'- -AGG-GAaGuUGGUGGUGGCGuuACGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 27175 | 0.69 | 0.510951 |
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Target: 5'- cCCCUUCGACgACgAuCCGCAcuccGUGGg -3' miRNA: 3'- aGGGAAGUUGgUGgU-GGCGUua--CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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