Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11894 | 3' | -53.9 | NC_003266.2 | + | 27175 | 0.69 | 0.510951 |
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Target: 5'- cCCCUUCGACgACgAuCCGCAcuccGUGGg -3' miRNA: 3'- aGGGAAGUUGgUGgU-GGCGUua--CGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 16154 | 0.77 | 0.166908 |
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Target: 5'- aUCCUUC-ACCGCCGCCGCGggGCc- -3' miRNA: 3'- aGGGAAGuUGGUGGUGGCGUuaCGcc -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 4840 | 0.72 | 0.319246 |
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Target: 5'- cUCCC-UCAACUACCACCuGCAGccggucauUGgGGu -3' miRNA: 3'- -AGGGaAGUUGGUGGUGG-CGUU--------ACgCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 26019 | 0.72 | 0.352978 |
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Target: 5'- gUCCaCUgCcGCCGCCGCCGC--UGUGGa -3' miRNA: 3'- -AGG-GAaGuUGGUGGUGGCGuuACGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 10255 | 0.71 | 0.370765 |
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Target: 5'- uUCCCUcgcgccUCcACCACCGCCGCcg-GCa- -3' miRNA: 3'- -AGGGA------AGuUGGUGGUGGCGuuaCGcc -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 14839 | 0.71 | 0.39857 |
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Target: 5'- -gCCUUgGccGCCGCCACUGC--UGCGGc -3' miRNA: 3'- agGGAAgU--UGGUGGUGGCGuuACGCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 17259 | 0.7 | 0.451844 |
|
Target: 5'- cUCCCccCAACCgcuugcugauggcgcGCCGCCGCcGGUGgGGg -3' miRNA: 3'- -AGGGaaGUUGG---------------UGGUGGCG-UUACgCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 17305 | 0.69 | 0.489442 |
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Target: 5'- gCCC--CGAUCGCCGCgGCGAUGaUGGg -3' miRNA: 3'- aGGGaaGUUGGUGGUGgCGUUAC-GCC- -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 10204 | 0.69 | 0.500145 |
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Target: 5'- -aUCUaCGGCUACCACCGCAccAUGCu- -3' miRNA: 3'- agGGAaGUUGGUGGUGGCGU--UACGcc -5' |
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| 11894 | 3' | -53.9 | NC_003266.2 | + | 22991 | 0.66 | 0.691237 |
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Target: 5'- --gCUUCAGCUACCcCC-CcGUGCGGa -3' miRNA: 3'- aggGAAGUUGGUGGuGGcGuUACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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