miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1198 3' -58.9 NC_001132.2 + 127642 0.67 0.832104
Target:  5'- aCgCCGACGAggaUCGUCGGgGUUCu- -3'
miRNA:   3'- -GgGGCUGCUaggAGCAGCCgCAGGcu -5'
1198 3' -58.9 NC_001132.2 + 127687 0.67 0.832104
Target:  5'- aCgCCGACGAggaUCGUCGGgGUUCu- -3'
miRNA:   3'- -GgGGCUGCUaggAGCAGCCgCAGGcu -5'
1198 3' -58.9 NC_001132.2 + 159929 0.68 0.7712
Target:  5'- aCCCCGuCGcucGUCCUCGguauaguacgcaCGGCGUCUc- -3'
miRNA:   3'- -GGGGCuGC---UAGGAGCa-----------GCCGCAGGcu -5'
1198 3' -58.9 NC_001132.2 + 159728 0.68 0.764022
Target:  5'- aCUCCGACGuuuuaaccaCUUCGUUGG-GUCCGGc -3'
miRNA:   3'- -GGGGCUGCua-------GGAGCAGCCgCAGGCU- -5'
1198 3' -58.9 NC_001132.2 + 157460 0.68 0.754956
Target:  5'- gCCCCGACGcguacacggCUUC-UCGGaCGUCCGu -3'
miRNA:   3'- -GGGGCUGCua-------GGAGcAGCC-GCAGGCu -5'
1198 3' -58.9 NC_001132.2 + 10790 0.68 0.754956
Target:  5'- uCCCgGGCGGaCUUCGgucaGGCGUCCc- -3'
miRNA:   3'- -GGGgCUGCUaGGAGCag--CCGCAGGcu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.