Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12097 | 5' | -56.9 | NC_003309.1 | + | 45272 | 0.66 | 0.650099 |
Target: 5'- aGCGGAUuugGUCGaAUGCCGGcCGCCgCGu -3' miRNA: 3'- cUGCUUGua-CGGC-UACGGCU-GCGG-GC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 17507 | 0.66 | 0.650099 |
Target: 5'- gGACGucagcGGCAcGCCGAcGCCGgccucACGCUCGa -3' miRNA: 3'- -CUGC-----UUGUaCGGCUaCGGC-----UGCGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 11502 | 0.66 | 0.639233 |
Target: 5'- cGCGAGgcucgcCGUGUCGAUcgcGCCGGCgGCUCGa -3' miRNA: 3'- cUGCUU------GUACGGCUA---CGGCUG-CGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 20152 | 0.66 | 0.639233 |
Target: 5'- cGACGAc---GCCGuuuucguUGUCGAUGCCCa -3' miRNA: 3'- -CUGCUuguaCGGCu------ACGGCUGCGGGc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 42660 | 0.66 | 0.639233 |
Target: 5'- aGCGAACggGCCGgcGauGGCGUCCu -3' miRNA: 3'- cUGCUUGuaCGGCuaCggCUGCGGGc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 31733 | 0.66 | 0.628362 |
Target: 5'- cGAUGAGCGUGagug-GCCGugGCCg- -3' miRNA: 3'- -CUGCUUGUACggcuaCGGCugCGGgc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 8944 | 0.66 | 0.617494 |
Target: 5'- cGACGAugcgguUGUGCaCGggGCCGAaCGCCUc -3' miRNA: 3'- -CUGCUu-----GUACG-GCuaCGGCU-GCGGGc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 17061 | 0.66 | 0.617494 |
Target: 5'- uACG-ACGUGCCGuUGuuGGCcguccccgcgaGCCCGg -3' miRNA: 3'- cUGCuUGUACGGCuACggCUG-----------CGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 37327 | 0.66 | 0.606639 |
Target: 5'- cACGGGCGUcuaccugcGCgCGAucgUGCgCGACGCCCu -3' miRNA: 3'- cUGCUUGUA--------CG-GCU---ACG-GCUGCGGGc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 1377 | 0.66 | 0.595805 |
Target: 5'- -uCGAGCAUuUCGAucaccUGCCGGCGCuuGu -3' miRNA: 3'- cuGCUUGUAcGGCU-----ACGGCUGCGggC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 5066 | 0.67 | 0.563516 |
Target: 5'- cACGGGCGcggcgGCCGGUGCCGcgacgGCGgUCGg -3' miRNA: 3'- cUGCUUGUa----CGGCUACGGC-----UGCgGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 45677 | 0.67 | 0.563516 |
Target: 5'- --aGGACGuUGCCcuUGcCCGACGCCCc -3' miRNA: 3'- cugCUUGU-ACGGcuAC-GGCUGCGGGc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 18701 | 0.67 | 0.542247 |
Target: 5'- cGCGGAUAcggccGCCGAU-CCGGCGgCCGg -3' miRNA: 3'- cUGCUUGUa----CGGCUAcGGCUGCgGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 10332 | 0.68 | 0.530662 |
Target: 5'- cGACGAGCGcucGCCGGgcGCCGAcccacgcugucgcCGCCUGc -3' miRNA: 3'- -CUGCUUGUa--CGGCUa-CGGCU-------------GCGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 44154 | 0.68 | 0.521251 |
Target: 5'- cGACGAGuccgcuuuuCGUGCggUGAUGCuCGuCGCCCGc -3' miRNA: 3'- -CUGCUU---------GUACG--GCUACG-GCuGCGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 22403 | 0.68 | 0.510871 |
Target: 5'- aACGcuucGGCAUGCCGcUGCgCGucgaACGCCCGc -3' miRNA: 3'- cUGC----UUGUACGGCuACG-GC----UGCGGGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 27329 | 0.68 | 0.500578 |
Target: 5'- cGGCGAAC-UGCCGAUGCaucaGAcuggauCGCCa- -3' miRNA: 3'- -CUGCUUGuACGGCUACGg---CU------GCGGgc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 16772 | 0.68 | 0.500578 |
Target: 5'- cGGCGAACGUgagGCCGccGUCG-CGCgCCGa -3' miRNA: 3'- -CUGCUUGUA---CGGCuaCGGCuGCG-GGC- -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 15234 | 0.68 | 0.499554 |
Target: 5'- cGACuGAAgAUGCCGAUgaaagcgcagcacGUCGGCGCCg- -3' miRNA: 3'- -CUG-CUUgUACGGCUA-------------CGGCUGCGGgc -5' |
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12097 | 5' | -56.9 | NC_003309.1 | + | 8186 | 0.68 | 0.489363 |
Target: 5'- cGACGAACAccagaucUGCCGc-GUCGAgGUCCGu -3' miRNA: 3'- -CUGCUUGU-------ACGGCuaCGGCUgCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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