Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12102 | 5' | -60.3 | NC_003309.1 | + | 46151 | 0.69 | 0.322265 |
Target: 5'- aCCGCCAuGCagcagUCGAGCagcuucaaagcagaGCGCaCGGUCGg -3' miRNA: 3'- aGGCGGU-CG-----AGCUUG--------------CGCG-GCCAGCg -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 45770 | 0.78 | 0.07537 |
Target: 5'- aCCGUCuucgGGUUCGGcgccugauguACGCGCCGGUCGUa -3' miRNA: 3'- aGGCGG----UCGAGCU----------UGCGCGGCCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 22747 | 0.71 | 0.224977 |
Target: 5'- gCCGUCAGUuugUCGAucuCGCGCUGaucGUCGCc -3' miRNA: 3'- aGGCGGUCG---AGCUu--GCGCGGC---CAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10612 | 0.73 | 0.156652 |
Target: 5'- aCUGCCGGCaacguUCGcGCGUGCC-GUCGCg -3' miRNA: 3'- aGGCGGUCG-----AGCuUGCGCGGcCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 22440 | 0.72 | 0.18329 |
Target: 5'- gCUGCCGGCgUCGAGCGCGgCG--CGCg -3' miRNA: 3'- aGGCGGUCG-AGCUUGCGCgGCcaGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 43529 | 0.72 | 0.193024 |
Target: 5'- gCCGUCGGCggccaucGCGCGCaucaGGUCGCg -3' miRNA: 3'- aGGCGGUCGagcu---UGCGCGg---CCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 31529 | 0.76 | 0.096534 |
Target: 5'- -gCGCCAGCUCGAACuaCGCCGGccaGCc -3' miRNA: 3'- agGCGGUCGAGCUUGc-GCGGCCag-CG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 42146 | 0.69 | 0.302275 |
Target: 5'- cCUGcCCAGCUCGGaaGCGCGCguugacgcucUGGaCGCa -3' miRNA: 3'- aGGC-GGUCGAGCU--UGCGCG----------GCCaGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 11744 | 0.69 | 0.309566 |
Target: 5'- cCCGCCAuGCg-GAccuGCGCGUCGuUCGCg -3' miRNA: 3'- aGGCGGU-CGagCU---UGCGCGGCcAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 7618 | 0.69 | 0.316241 |
Target: 5'- cUCgGCCucGC-CGAucgACGCaucaaacGCCGGUCGCa -3' miRNA: 3'- -AGgCGGu-CGaGCU---UGCG-------CGGCCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 11495 | 0.69 | 0.286011 |
Target: 5'- cUCGCCGugUCGAuCGCGCCGGcggcucgaacaaguUCGCa -3' miRNA: 3'- aGGCGGUcgAGCUuGCGCGGCC--------------AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 7807 | 0.69 | 0.324546 |
Target: 5'- -gCGCCAugUCGAGCGCGCCauGUaCGCg -3' miRNA: 3'- agGCGGUcgAGCUUGCGCGGc-CA-GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 22645 | 0.69 | 0.324546 |
Target: 5'- -aCGCCGGCggcgcUGGAaGCGCCGGgcaCGCc -3' miRNA: 3'- agGCGGUCGa----GCUUgCGCGGCCa--GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 5573 | 0.69 | 0.324546 |
Target: 5'- -aCGCCuuGUcuUCGcGCGCGCCGaUCGCg -3' miRNA: 3'- agGCGGu-CG--AGCuUGCGCGGCcAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10873 | 0.68 | 0.332237 |
Target: 5'- uUCCGCUcGCUC--GCGCGCCuGcGUgGCa -3' miRNA: 3'- -AGGCGGuCGAGcuUGCGCGG-C-CAgCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 11988 | 0.68 | 0.34006 |
Target: 5'- cCCGCgcgcggCAGCcgCGGcGCGCGCCGcGUCGg -3' miRNA: 3'- aGGCG------GUCGa-GCU-UGCGCGGC-CAGCg -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10569 | 0.68 | 0.348016 |
Target: 5'- gCCGCCGcCUCGAcggcACGCGCuuCGGcaagcuUCGCu -3' miRNA: 3'- aGGCGGUcGAGCU----UGCGCG--GCC------AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 5173 | 0.68 | 0.356104 |
Target: 5'- aUCGCCAGuUUCGAggcgagcuGCGCGUCGccgCGCg -3' miRNA: 3'- aGGCGGUC-GAGCU--------UGCGCGGCca-GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 19038 | 0.67 | 0.381152 |
Target: 5'- aUCgGCCGuuGCUgGAucuGCGCGCC-GUCGUc -3' miRNA: 3'- -AGgCGGU--CGAgCU---UGCGCGGcCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 18715 | 0.67 | 0.381152 |
Target: 5'- cUCGCCGGCUgCG-GCGCGgauaCGGcCGCc -3' miRNA: 3'- aGGCGGUCGA-GCuUGCGCg---GCCaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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