Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12112 | 5' | -54.4 | NC_003309.1 | + | 22453 | 0.66 | 0.752654 |
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Target: 5'- gCGGCGAuCGCacg--CUGCCGgCGUCg -3' miRNA: 3'- aGCUGCUuGUGgucuaGACGGC-GCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 966 | 0.66 | 0.752654 |
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Target: 5'- gCGGCGGuCACC-GAUUUGCCGUu-- -3' miRNA: 3'- aGCUGCUuGUGGuCUAGACGGCGcag -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 32387 | 0.66 | 0.76299 |
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Target: 5'- gCGACGGcucGCugCGGcg--GCCGUGUCc -3' miRNA: 3'- aGCUGCU---UGugGUCuagaCGGCGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 13162 | 0.66 | 0.773191 |
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Target: 5'- gCGGCGAAUcgcgcgaaGCCGGAUC-GCCccccuGCGUUc -3' miRNA: 3'- aGCUGCUUG--------UGGUCUAGaCGG-----CGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 33965 | 0.66 | 0.773191 |
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Target: 5'- aCGACGAuUACC-GAUgUGaCCGCGcCg -3' miRNA: 3'- aGCUGCUuGUGGuCUAgAC-GGCGCaG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 30876 | 0.66 | 0.777231 |
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Target: 5'- gCGAuCGAACcuagucgcaauccgaACgAGAUCaacgGCCGUGUCa -3' miRNA: 3'- aGCU-GCUUG---------------UGgUCUAGa---CGGCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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