Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12112 | 5' | -54.4 | NC_003309.1 | + | 32809 | 0.69 | 0.589094 |
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Target: 5'- aCGACGAGuCGCUGGAgauggCUGCUgauGCGUUg -3' miRNA: 3'- aGCUGCUU-GUGGUCUa----GACGG---CGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 33965 | 0.66 | 0.773191 |
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Target: 5'- aCGACGAuUACC-GAUgUGaCCGCGcCg -3' miRNA: 3'- aGCUGCUuGUGGuCUAgAC-GGCGCaG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 43526 | 0.67 | 0.71018 |
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Target: 5'- gUCGGCGGccaucgcgcGCAUCAGGUC-GCgGCaGUCu -3' miRNA: 3'- -AGCUGCU---------UGUGGUCUAGaCGgCG-CAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 46365 | 0.68 | 0.655425 |
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Target: 5'- cUCcGCGAGCGCCGccucGAUCUGCuuaCGCGg- -3' miRNA: 3'- -AGcUGCUUGUGGU----CUAGACG---GCGCag -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 48725 | 0.68 | 0.666463 |
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Target: 5'- --aGCGAAuCAUCAGGUCgacugccaaUGCCGCGUg -3' miRNA: 3'- agcUGCUU-GUGGUCUAG---------ACGGCGCAg -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 52577 | 0.69 | 0.589094 |
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Target: 5'- -gGACGGcCACCGGGccUUUGCCGUGg- -3' miRNA: 3'- agCUGCUuGUGGUCU--AGACGGCGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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