Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12112 | 5' | -54.4 | NC_003309.1 | + | 20024 | 0.66 | 0.742192 |
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Target: 5'- gCGACGGuuuGCACCg---CcGCCGCGUUg -3' miRNA: 3'- aGCUGCU---UGUGGucuaGaCGGCGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 14868 | 0.66 | 0.731618 |
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Target: 5'- gCGAUG-ACGCCAGc-CUGCCGCc-- -3' miRNA: 3'- aGCUGCuUGUGGUCuaGACGGCGcag -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 15329 | 0.67 | 0.720944 |
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Target: 5'- gUCGGCGAugGCUg---CUGC-GCGUCg -3' miRNA: 3'- -AGCUGCUugUGGucuaGACGgCGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 11904 | 0.67 | 0.716648 |
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Target: 5'- cUCGGCGAGCGCCuccgcaaccaccAUCUGCUGauUCa -3' miRNA: 3'- -AGCUGCUUGUGGuc----------UAGACGGCgcAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 43526 | 0.67 | 0.71018 |
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Target: 5'- gUCGGCGGccaucgcgcGCAUCAGGUC-GCgGCaGUCu -3' miRNA: 3'- -AGCUGCU---------UGUGGUCUAGaCGgCG-CAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 8188 | 1.11 | 0.000875 |
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Target: 5'- aUCGACGAACACCAGAUCUGCCGCGUCg -3' miRNA: 3'- -AGCUGCUUGUGGUCUAGACGGCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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