Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12112 | 5' | -54.4 | NC_003309.1 | + | 11234 | 0.67 | 0.699338 |
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Target: 5'- aCG-CGGACugCGcgucGUCUGCCGCG-Cg -3' miRNA: 3'- aGCuGCUUGugGUc---UAGACGGCGCaG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 10334 | 0.69 | 0.571546 |
|
Target: 5'- cUCGACGAGCgcucGCCGGGcgccgacccacgcugUCgccGCCuGCGUCg -3' miRNA: 3'- -AGCUGCUUG----UGGUCU---------------AGa--CGG-CGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 8188 | 1.11 | 0.000875 |
|
Target: 5'- aUCGACGAACACCAGAUCUGCCGCGUCg -3' miRNA: 3'- -AGCUGCUUGUGGUCUAGACGGCGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 7814 | 0.71 | 0.472018 |
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Target: 5'- cCGGCGAGCGCCAuGUCgaGCgcgccauguacgCGCGUCa -3' miRNA: 3'- aGCUGCUUGUGGUcUAGa-CG------------GCGCAG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 5160 | 0.66 | 0.742192 |
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Target: 5'- -aGGCGAGCugCGcGUC-GCCGCG-Cg -3' miRNA: 3'- agCUGCUUGugGUcUAGaCGGCGCaG- -5' |
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| 12112 | 5' | -54.4 | NC_003309.1 | + | 966 | 0.66 | 0.752654 |
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Target: 5'- gCGGCGGuCACC-GAUUUGCCGUu-- -3' miRNA: 3'- aGCUGCUuGUGGuCUAGACGGCGcag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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