Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12119 | 3' | -57.8 | NC_003309.1 | + | 6509 | 0.66 | 0.531711 |
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Target: 5'- -cGCUUGCCgCG-CGCCcGgGAucACGACGc -3' miRNA: 3'- cuCGAACGG-GCuGCGG-CgCU--UGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 22059 | 0.67 | 0.521251 |
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Target: 5'- aAGCUca-CCGcCGCCGCGAGCuGAUAu -3' miRNA: 3'- cUCGAacgGGCuGCGGCGCUUG-CUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 10694 | 0.67 | 0.510871 |
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Target: 5'- cGAGCUccugcUGCgCGGCGgccgucUCGCGGGCGAUg -3' miRNA: 3'- -CUCGA-----ACGgGCUGC------GGCGCUUGCUGu -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 46287 | 0.67 | 0.500578 |
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Target: 5'- cGAGCgcgcgUGCCCuuccuUGCCGUcAGCGGCAu -3' miRNA: 3'- -CUCGa----ACGGGcu---GCGGCGcUUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 47502 | 0.67 | 0.490378 |
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Target: 5'- cAGCUU-CUCGAgGCgGCGcAGCGACAc -3' miRNA: 3'- cUCGAAcGGGCUgCGgCGC-UUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 10829 | 0.67 | 0.489363 |
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Target: 5'- cGGCUcGCgCGAgGCCGUGAuccgucucggccaGCGACGc -3' miRNA: 3'- cUCGAaCGgGCUgCGGCGCU-------------UGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 12804 | 1.07 | 0.000689 |
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Target: 5'- uGAGCUUGCCCGACGCCGCGAACGACAc -3' miRNA: 3'- -CUCGAACGGGCUGCGGCGCUUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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