Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12119 | 3' | -57.8 | NC_003309.1 | + | 52445 | 0.66 | 0.531711 |
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Target: 5'- -cGCUgUGCUCGAUGCUgaacugGCGGGCGAUc -3' miRNA: 3'- cuCGA-ACGGGCUGCGG------CGCUUGCUGu -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 6509 | 0.66 | 0.531711 |
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Target: 5'- -cGCUUGCCgCG-CGCCcGgGAucACGACGc -3' miRNA: 3'- cuCGAACGG-GCuGCGG-CgCU--UGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 31730 | 0.66 | 0.542247 |
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Target: 5'- uGAGCgugagUGgCCGugGCCGCaugucuggcucgGcGCGACGa -3' miRNA: 3'- -CUCGa----ACgGGCugCGGCG------------CuUGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 18790 | 0.66 | 0.552851 |
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Target: 5'- -cGCgaUGCCCGAcggcaagauCGCCGUGAaacgaucgccgGCGGCGa -3' miRNA: 3'- cuCGa-ACGGGCU---------GCGGCGCU-----------UGCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 48225 | 0.66 | 0.552851 |
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Target: 5'- cGAGCc-GUCCGAgCGCCGCuGACGcGCGa -3' miRNA: 3'- -CUCGaaCGGGCU-GCGGCGcUUGC-UGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 15426 | 0.66 | 0.563516 |
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Target: 5'- -cGCgagUGUCCGGCGCCG-GAAcaccuucgcgcCGACGa -3' miRNA: 3'- cuCGa--ACGGGCUGCGGCgCUU-----------GCUGU- -5' |
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| 12119 | 3' | -57.8 | NC_003309.1 | + | 44405 | 0.66 | 0.574235 |
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Target: 5'- cGAGCgccucggggaagUUGCaaagugGGCGCCGuCGAACGACu -3' miRNA: 3'- -CUCG------------AACGgg----CUGCGGC-GCUUGCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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