Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12119 | 3' | -57.8 | NC_003309.1 | + | 34003 | 0.69 | 0.384654 |
Target: 5'- aGGGCUUGCgUGGCGCaguuCGCGGuaagcguguucgcACGACGa -3' miRNA: 3'- -CUCGAACGgGCUGCG----GCGCU-------------UGCUGU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 3399 | 0.7 | 0.33494 |
Target: 5'- gGAGCgacuggUGCUCGAUGuuGCuGAACGugGc -3' miRNA: 3'- -CUCGa-----ACGGGCUGCggCG-CUUGCugU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 5157 | 0.7 | 0.326993 |
Target: 5'- cGAGCUgcgcgucGCCgCGcgcCGCCGCGAGCGcACGu -3' miRNA: 3'- -CUCGAa------CGG-GCu--GCGGCGCUUGC-UGU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 9984 | 0.73 | 0.224465 |
Target: 5'- uGAGCUgcgccgaacucuUGCCC-AUGCCGCGcGCGAUAg -3' miRNA: 3'- -CUCGA------------ACGGGcUGCGGCGCuUGCUGU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 10257 | 0.73 | 0.207557 |
Target: 5'- cGGCgauccGCCCa--GCCGCGAGCGACAc -3' miRNA: 3'- cUCGaa---CGGGcugCGGCGCUUGCUGU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 21980 | 0.75 | 0.163313 |
Target: 5'- uGAGCcgUUGCUCGA-GCCGCGcGCGGCAu -3' miRNA: 3'- -CUCG--AACGGGCUgCGGCGCuUGCUGU- -5' |
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12119 | 3' | -57.8 | NC_003309.1 | + | 12804 | 1.07 | 0.000689 |
Target: 5'- uGAGCUUGCCCGACGCCGCGAACGACAc -3' miRNA: 3'- -CUCGAACGGGCUGCGGCGCUUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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