Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12126 | 5' | -63.8 | NC_003309.1 | + | 22699 | 0.66 | 0.302159 |
Target: 5'- aCgCGUUCCGCaACCGC-UGCGUCGAc -3' miRNA: 3'- aGgGCGGGGUG-UGGCGcGCGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 16958 | 0.66 | 0.295099 |
Target: 5'- aUgCGCCCCACgGCUGCGCGaaaccgaaCGUCGc- -3' miRNA: 3'- aGgGCGGGGUG-UGGCGCGC--------GCAGCua -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 47565 | 0.66 | 0.288168 |
Target: 5'- cCCCGUCCa--GCCGCGCaauaccgcuuCGUCGAUg -3' miRNA: 3'- aGGGCGGGgugUGGCGCGc---------GCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 12613 | 0.66 | 0.288168 |
Target: 5'- aCCUGCUCCGCcucgGCCGUGUugauGCGcUCGAUc -3' miRNA: 3'- aGGGCGGGGUG----UGGCGCG----CGC-AGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 25392 | 0.66 | 0.288168 |
Target: 5'- cCUCGUgCCACugCGUGUagccgucgGCGUCGGUc -3' miRNA: 3'- aGGGCGgGGUGugGCGCG--------CGCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 10441 | 0.66 | 0.281366 |
Target: 5'- -gCCGCCUC-CGCCGCcuGCGCGUUu-- -3' miRNA: 3'- agGGCGGGGuGUGGCG--CGCGCAGcua -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 18879 | 0.67 | 0.274692 |
Target: 5'- aUCCacacagCGCCCgGCACCG-GaucaGCGUCGAa -3' miRNA: 3'- -AGG------GCGGGgUGUGGCgCg---CGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 15345 | 0.67 | 0.274032 |
Target: 5'- gUCCCcCaCCCGCagcgucggcgaugGCUGCuGCGCGUCGGa -3' miRNA: 3'- -AGGGcG-GGGUG-------------UGGCG-CGCGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 17319 | 0.67 | 0.268145 |
Target: 5'- cCCCGCCgagaUCACAucgagcaccuUCGCGCGCGcaaUCGAa -3' miRNA: 3'- aGGGCGG----GGUGU----------GGCGCGCGC---AGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 37970 | 0.67 | 0.261725 |
Target: 5'- -aCCGCaguucCCCGCucGCCGCGCGCGg-GAUu -3' miRNA: 3'- agGGCG-----GGGUG--UGGCGCGCGCagCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 11996 | 0.67 | 0.257933 |
Target: 5'- gCCUGCugCCCGCGCgcggcagccgcggcgCGCGcCGCGUCGGc -3' miRNA: 3'- aGGGCG--GGGUGUG---------------GCGC-GCGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 13019 | 0.67 | 0.25543 |
Target: 5'- gCCCGguCCCCACACCGCccCGCcuGUCGc- -3' miRNA: 3'- aGGGC--GGGGUGUGGCGc-GCG--CAGCua -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 16549 | 1.06 | 0.000259 |
Target: 5'- aUCCCGCCCCACACCGCGCGCGUCGAUa -3' miRNA: 3'- -AGGGCGGGGUGUGGCGCGCGCAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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