Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12126 | 5' | -63.8 | NC_003309.1 | + | 22699 | 0.66 | 0.302159 |
Target: 5'- aCgCGUUCCGCaACCGC-UGCGUCGAc -3' miRNA: 3'- aGgGCGGGGUG-UGGCGcGCGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 25392 | 0.66 | 0.288168 |
Target: 5'- cCUCGUgCCACugCGUGUagccgucgGCGUCGGUc -3' miRNA: 3'- aGGGCGgGGUGugGCGCG--------CGCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 32566 | 0.7 | 0.153976 |
Target: 5'- aCCUGCUCUACGCCGaCGCaaaguCGUCGAc -3' miRNA: 3'- aGGGCGGGGUGUGGC-GCGc----GCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 32991 | 0.71 | 0.142352 |
Target: 5'- aUCCGCaucuucgcagCCCGCACCGCGCGCugaggcgCGAg -3' miRNA: 3'- aGGGCG----------GGGUGUGGCGCGCGca-----GCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 37295 | 0.69 | 0.179831 |
Target: 5'- --aCGCCCUgaaucuCGCCG-GCGCGUCGAUg -3' miRNA: 3'- aggGCGGGGu-----GUGGCgCGCGCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 37970 | 0.67 | 0.261725 |
Target: 5'- -aCCGCaguucCCCGCucGCCGCGCGCGg-GAUu -3' miRNA: 3'- agGGCG-----GGGUG--UGGCGCGCGCagCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 39969 | 0.7 | 0.150009 |
Target: 5'- gUCUGCCUCGCGgCGCGCuuGUCGAg -3' miRNA: 3'- aGGGCGGGGUGUgGCGCGcgCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 42153 | 0.69 | 0.189272 |
Target: 5'- aCCgGCCCC-UGCCcagcucggaaGCGCGCGUUGAc -3' miRNA: 3'- aGGgCGGGGuGUGG----------CGCGCGCAGCUa -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 45098 | 0.72 | 0.115182 |
Target: 5'- cCCUGCCacgaCCACACCGCGUaaccGCgGUCGAUc -3' miRNA: 3'- aGGGCGG----GGUGUGGCGCG----CG-CAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 45909 | 0.71 | 0.127089 |
Target: 5'- gCCCGCCUCGauCAguugacgguucagcUCGCGCGCGUUGAUc -3' miRNA: 3'- aGGGCGGGGU--GU--------------GGCGCGCGCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 47343 | 0.72 | 0.112147 |
Target: 5'- aCUCGCggCgCACGCCGCGCGgGUUGAUg -3' miRNA: 3'- aGGGCG--GgGUGUGGCGCGCgCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 47565 | 0.66 | 0.288168 |
Target: 5'- cCCCGUCCa--GCCGCGCaauaccgcuuCGUCGAUg -3' miRNA: 3'- aGGGCGGGgugUGGCGCGc---------GCAGCUA- -5' |
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12126 | 5' | -63.8 | NC_003309.1 | + | 47610 | 0.67 | 0.24926 |
Target: 5'- gUCCUGCCCgGucguCGCCGCGC-CGUCc-- -3' miRNA: 3'- -AGGGCGGGgU----GUGGCGCGcGCAGcua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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