Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12134 | 3' | -58.5 | NC_003309.1 | + | 15014 | 0.69 | 0.396424 |
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Target: 5'- -aGGUCAGCGACGgcCGccccuUCGACGGCc- -3' miRNA: 3'- cgCCAGUCGCUGU--GCu----GGCUGCCGcc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 22493 | 0.7 | 0.370764 |
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Target: 5'- uUGGUCgacGGCGGcCACcGCCGacGCGGCGGc -3' miRNA: 3'- cGCCAG---UCGCU-GUGcUGGC--UGCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 46112 | 0.71 | 0.308057 |
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Target: 5'- aCGGUCGGCaccucGACAcCGAacgCGACGGCGa -3' miRNA: 3'- cGCCAGUCG-----CUGU-GCUg--GCUGCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 5057 | 0.72 | 0.273157 |
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Target: 5'- gGCGGcCGGUGcCGCGA-CGGCGGuCGGa -3' miRNA: 3'- -CGCCaGUCGCuGUGCUgGCUGCC-GCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 8554 | 0.72 | 0.273157 |
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Target: 5'- uGCGaUCGGgGAguUACGccGCCGGCGGCGGc -3' miRNA: 3'- -CGCcAGUCgCU--GUGC--UGGCUGCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 9647 | 0.72 | 0.273157 |
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Target: 5'- cUGGUCAcGCGugACGAuCCGAaGGCGa -3' miRNA: 3'- cGCCAGU-CGCugUGCU-GGCUgCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 26774 | 0.74 | 0.218427 |
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Target: 5'- aGCGGUCGGgGAaa-GGuuGACGGCGa -3' miRNA: 3'- -CGCCAGUCgCUgugCUggCUGCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 17513 | 0.76 | 0.148083 |
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Target: 5'- cGCGucggacGUCAGCGGCACG-CCGACGcCGGc -3' miRNA: 3'- -CGC------CAGUCGCUGUGCuGGCUGCcGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 19384 | 1.11 | 0.000447 |
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Target: 5'- cGCGGUCAGCGACACGACCGACGGCGGc -3' miRNA: 3'- -CGCCAGUCGCUGUGCUGGCUGCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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