Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12139 | 3' | -55.2 | NC_003309.1 | + | 47395 | 0.71 | 0.348246 |
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Target: 5'- -cGACGGGCAACaGuCGgGCAGCuCGAUUg -3' miRNA: 3'- auCUGCUCGUUG-C-GCgCGUCG-GUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 5161 | 0.72 | 0.339788 |
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Target: 5'- gAGGCGAGC--UGCGCGUcGCCGcgCg -3' miRNA: 3'- aUCUGCUCGuuGCGCGCGuCGGUuaG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 5332 | 0.72 | 0.30746 |
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Target: 5'- gAGAgCG-GCAGCGUGCGCGcGCCGAg- -3' miRNA: 3'- aUCU-GCuCGUUGCGCGCGU-CGGUUag -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 1300 | 0.73 | 0.291451 |
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Target: 5'- --cAUGAGCGgcugucgACGCGCGCccauGCCAGUCa -3' miRNA: 3'- aucUGCUCGU-------UGCGCGCGu---CGGUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 23468 | 0.73 | 0.277536 |
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Target: 5'- gUAGAa---CAGCGUGUGCGGCCGAUCg -3' miRNA: 3'- -AUCUgcucGUUGCGCGCGUCGGUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 53305 | 0.74 | 0.249322 |
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Target: 5'- cGGGCGGGCAACGaCGgGCGgGCCAccaccacGUCa -3' miRNA: 3'- aUCUGCUCGUUGC-GCgCGU-CGGU-------UAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 43695 | 0.76 | 0.180691 |
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Target: 5'- -cGGCuAGCAGCGCGCGC-GCCAGaUCg -3' miRNA: 3'- auCUGcUCGUUGCGCGCGuCGGUU-AG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 23764 | 1.09 | 0.000786 |
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Target: 5'- aUAGACGAGCAACGCGCGCAGCCAAUCg -3' miRNA: 3'- -AUCUGCUCGUUGCGCGCGUCGGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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