Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12139 | 3' | -55.2 | NC_003309.1 | + | 39293 | 0.66 | 0.680992 |
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Target: 5'- cGGACGAugcguggcaacucgcGCAugcguugcgucgccaGCGCGCAGCCcgcgGAUCa -3' miRNA: 3'- aUCUGCU---------------CGUug-------------CGCGCGUCGG----UUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 43695 | 0.76 | 0.180691 |
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Target: 5'- -cGGCuAGCAGCGCGCGC-GCCAGaUCg -3' miRNA: 3'- auCUGcUCGUUGCGCGCGuCGGUU-AG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 45322 | 0.66 | 0.635015 |
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Target: 5'- -cGGCGuugauGCAugGCaUGCAGCCAAc- -3' miRNA: 3'- auCUGCu----CGUugCGcGCGUCGGUUag -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 47011 | 0.68 | 0.545773 |
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Target: 5'- -cGGCGAuuGGCGCGUcuucGCAGCCAacuuGUCg -3' miRNA: 3'- auCUGCUcgUUGCGCG----CGUCGGU----UAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 47395 | 0.71 | 0.348246 |
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Target: 5'- -cGACGGGCAACaGuCGgGCAGCuCGAUUg -3' miRNA: 3'- auCUGCUCGUUG-C-GCgCGUCG-GUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 47776 | 0.67 | 0.612523 |
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Target: 5'- --aGCGGGCAGCcuCGCGCAGCauuUCc -3' miRNA: 3'- aucUGCUCGUUGc-GCGCGUCGguuAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 49469 | 0.69 | 0.46092 |
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Target: 5'- -cGcCGAGC-ACGCGCGaauGCCGAUUg -3' miRNA: 3'- auCuGCUCGuUGCGCGCgu-CGGUUAG- -5' |
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| 12139 | 3' | -55.2 | NC_003309.1 | + | 53305 | 0.74 | 0.249322 |
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Target: 5'- cGGGCGGGCAACGaCGgGCGgGCCAccaccacGUCa -3' miRNA: 3'- aUCUGCUCGUUGC-GCgCGU-CGGU-------UAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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