Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12139 | 3' | -55.2 | NC_003309.1 | + | 47776 | 0.67 | 0.612523 |
Target: 5'- --aGCGGGCAGCcuCGCGCAGCauuUCc -3' miRNA: 3'- aucUGCUCGUUGc-GCGCGUCGguuAG- -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 45322 | 0.66 | 0.635015 |
Target: 5'- -cGGCGuugauGCAugGCaUGCAGCCAAc- -3' miRNA: 3'- auCUGCu----CGUugCGcGCGUCGGUUag -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 27798 | 0.66 | 0.635015 |
Target: 5'- cGGACGAGUGGCGUGCccGCgaagAGCUcaAGUCu -3' miRNA: 3'- aUCUGCUCGUUGCGCG--CG----UCGG--UUAG- -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 10645 | 0.66 | 0.668704 |
Target: 5'- cGGcAUGcccGCGAUGCGCGC-GCCGAUUu -3' miRNA: 3'- aUC-UGCu--CGUUGCGCGCGuCGGUUAG- -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 6797 | 0.66 | 0.668704 |
Target: 5'- -cGACGGGUAuCGCGCaaGGUCAAUg -3' miRNA: 3'- auCUGCUCGUuGCGCGcgUCGGUUAg -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 34535 | 0.66 | 0.678762 |
Target: 5'- gUAG-CGAGCAcccgucgAUGUGgGCGGCC-GUCg -3' miRNA: 3'- -AUCuGCUCGU-------UGCGCgCGUCGGuUAG- -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 21983 | 0.66 | 0.679877 |
Target: 5'- -cGAUGAGCcguugcucgAGcCGCGCGCGGCau-UCa -3' miRNA: 3'- auCUGCUCG---------UU-GCGCGCGUCGguuAG- -5' |
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12139 | 3' | -55.2 | NC_003309.1 | + | 39293 | 0.66 | 0.680992 |
Target: 5'- cGGACGAugcguggcaacucgcGCAugcguugcgucgccaGCGCGCAGCCcgcgGAUCa -3' miRNA: 3'- aUCUGCU---------------CGUug-------------CGCGCGUCGG----UUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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