Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12140 | 5' | -55.7 | NC_003309.1 | + | 43537 | 0.73 | 0.279193 |
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Target: 5'- aCG-CGCAcGCcgucgGCGGCCAUCGCGCGc -3' miRNA: 3'- aGCuGCGU-CGa----CGUCGGUAGUGUGCu -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 44179 | 0.67 | 0.576157 |
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Target: 5'- gCGGCGC-GCUGCcGCuCGgcgUACGCGAc -3' miRNA: 3'- aGCUGCGuCGACGuCG-GUa--GUGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 45318 | 0.77 | 0.164088 |
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Target: 5'- gUUGAUGCauGGCaUGCAGCCAaCGCGCGAc -3' miRNA: 3'- -AGCUGCG--UCG-ACGUCGGUaGUGUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 45869 | 0.68 | 0.554343 |
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Target: 5'- aUC-ACGCGGC-GaCGGCCGUCGauCACGAg -3' miRNA: 3'- -AGcUGCGUCGaC-GUCGGUAGU--GUGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 46786 | 0.68 | 0.554343 |
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Target: 5'- gCGugGCcGCUGCgcgAGCUGUCACcgcaACGGg -3' miRNA: 3'- aGCugCGuCGACG---UCGGUAGUG----UGCU- -5' |
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| 12140 | 5' | -55.7 | NC_003309.1 | + | 47948 | 0.7 | 0.420928 |
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Target: 5'- cUCGGCGaAGCUGCgaccgauaguucGGUCGUCGCGCa- -3' miRNA: 3'- -AGCUGCgUCGACG------------UCGGUAGUGUGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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