Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12211 | 3' | -59.1 | NC_003309.1 | + | 17514 | 0.65 | 0.550373 |
Target: 5'- gCGCGUcggacgucaGCGGCACGCcgacgccGGCcuCaCGCUCGa -3' miRNA: 3'- -GCGCG---------CGCUGUGUG-------CCGuuG-GCGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 22729 | 0.66 | 0.541017 |
Target: 5'- uCGCGCugaucgucgccgGCGACGC-CGaaaacgcguuccGCAACCGCUgCGu -3' miRNA: 3'- -GCGCG------------CGCUGUGuGC------------CGUUGGCGA-GC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 48217 | 0.66 | 0.541017 |
Target: 5'- cCGaGCGCcGCugACGcGCGAUCaGCUCGa -3' miRNA: 3'- -GCgCGCGcUGugUGC-CGUUGG-CGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 3142 | 0.66 | 0.541017 |
Target: 5'- aGCGC-CGGgGCAUccuuCGGCCGCUCGa -3' miRNA: 3'- gCGCGcGCUgUGUGcc--GUUGGCGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 47014 | 0.66 | 0.539981 |
Target: 5'- aCGCG-GCGAUugGCGcgucuucGCAGCCaacuugucgacGCUCGg -3' miRNA: 3'- -GCGCgCGCUGugUGC-------CGUUGG-----------CGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 7221 | 0.66 | 0.530684 |
Target: 5'- cCGCGUuuGCG-CGC-CGGCAguACCGC-CGu -3' miRNA: 3'- -GCGCG--CGCuGUGuGCCGU--UGGCGaGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 22084 | 0.66 | 0.530684 |
Target: 5'- gGCGCGCGGCcCGauguuguaGGCGAa-GCUCa -3' miRNA: 3'- gCGCGCGCUGuGUg-------CCGUUggCGAGc -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 39467 | 0.66 | 0.530684 |
Target: 5'- uCGUGCaGCGugGacUACuGCGGCCuGCUCGa -3' miRNA: 3'- -GCGCG-CGCugU--GUGcCGUUGG-CGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 24037 | 0.66 | 0.520425 |
Target: 5'- uGCGUGCucgACACGGCuccACCGCcCGu -3' miRNA: 3'- gCGCGCGcugUGUGCCGu--UGGCGaGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 43515 | 0.66 | 0.520425 |
Target: 5'- uCGCGCGCaucaGguCGCGGCAGucuCCGCggugCGa -3' miRNA: 3'- -GCGCGCGc---UguGUGCCGUU---GGCGa---GC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 9641 | 0.66 | 0.510244 |
Target: 5'- -aCGCGUGACGauccgaaGGCGAUUGCUCa -3' miRNA: 3'- gcGCGCGCUGUgug----CCGUUGGCGAGc -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 18797 | 0.66 | 0.510244 |
Target: 5'- gCGCcuGCGCGAUGCccgACGGCAagAUCGC-CGu -3' miRNA: 3'- -GCG--CGCGCUGUG---UGCCGU--UGGCGaGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 53289 | 0.66 | 0.500147 |
Target: 5'- gGCGgGCcACcacCACGucaccGCAACCGCUCGc -3' miRNA: 3'- gCGCgCGcUGu--GUGC-----CGUUGGCGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 32391 | 0.66 | 0.500147 |
Target: 5'- aGCG-GCGACGgcucgcUGCGGCGGCCGUg-- -3' miRNA: 3'- gCGCgCGCUGU------GUGCCGUUGGCGagc -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 21964 | 0.67 | 0.49014 |
Target: 5'- cCGCGCGCGGCAuucauCACGcGUGAagCGCUgGc -3' miRNA: 3'- -GCGCGCGCUGU-----GUGC-CGUUg-GCGAgC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 32054 | 0.67 | 0.49014 |
Target: 5'- gGCGCGCaAUGCACGcuucGCuGGCgGCUCGg -3' miRNA: 3'- gCGCGCGcUGUGUGC----CG-UUGgCGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 5562 | 0.67 | 0.480226 |
Target: 5'- uCGCGCGCGcCGauCGCGGCGGugaggUCGC-CGa -3' miRNA: 3'- -GCGCGCGCuGU--GUGCCGUU-----GGCGaGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 30992 | 0.67 | 0.480226 |
Target: 5'- uCGCGaGCGGCuACAaccGCAACCGCgugCGa -3' miRNA: 3'- -GCGCgCGCUG-UGUgc-CGUUGGCGa--GC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 47831 | 0.67 | 0.480226 |
Target: 5'- uCGCGCGgGAUgucguugaaguAC-CGGUAcaACUGCUCGu -3' miRNA: 3'- -GCGCGCgCUG-----------UGuGCCGU--UGGCGAGC- -5' |
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12211 | 3' | -59.1 | NC_003309.1 | + | 17793 | 0.67 | 0.474326 |
Target: 5'- gCGCGUucgGCGugAUACGGCGcacacggaucagccaGCCGUUUu -3' miRNA: 3'- -GCGCG---CGCugUGUGCCGU---------------UGGCGAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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