Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12216 | 5' | -50.7 | NC_003309.1 | + | 45353 | 0.66 | 0.933158 |
Target: 5'- cGGCGCGCGAucucucgcaguuCCAACuuccCGGCGu--- -3' miRNA: 3'- -CCGCGCGCUu-----------GGUUGua--GCUGUuaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 22713 | 0.66 | 0.932599 |
Target: 5'- cGGCGaCGcCGAaaacgcguuccgcaACCGcuGCGUCGAC-GUCUg -3' miRNA: 3'- -CCGC-GC-GCU--------------UGGU--UGUAGCUGuUAGA- -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 31775 | 0.66 | 0.928608 |
Target: 5'- cGCGCGgGAuaugauuuCCAGCGuccUCGGCAgcAUCa -3' miRNA: 3'- cCGCGCgCUu-------GGUUGU---AGCUGU--UAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 44771 | 0.66 | 0.928608 |
Target: 5'- aGCGCGCGAGgCAAauaGUCGAgCGcgUa -3' miRNA: 3'- cCGCGCGCUUgGUUg--UAGCU-GUuaGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 29942 | 0.66 | 0.912524 |
Target: 5'- uGCGCGCGuuccuguCCGguuucuuccagugguGCGUCGACAAg-- -3' miRNA: 3'- cCGCGCGCuu-----GGU---------------UGUAGCUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 29584 | 0.66 | 0.909875 |
Target: 5'- cGGCGUGCGggUCGAUuUCaa-GAUCa -3' miRNA: 3'- -CCGCGCGCuuGGUUGuAGcugUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 4183 | 0.66 | 0.934266 |
Target: 5'- aGGCaGCGCGGaugucguccGCCAGC-UCGAacgcgcCGGUCg -3' miRNA: 3'- -CCG-CGCGCU---------UGGUUGuAGCU------GUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 43178 | 0.67 | 0.903049 |
Target: 5'- cGuCGCGCGAACC-GCGUCaGCGAg-- -3' miRNA: 3'- cC-GCGCGCUUGGuUGUAGcUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 11828 | 0.67 | 0.87293 |
Target: 5'- cGCGCGCccGCCGcgGCuuucgaauccuuGUCGGCGAUCg -3' miRNA: 3'- cCGCGCGcuUGGU--UG------------UAGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 50233 | 0.67 | 0.888544 |
Target: 5'- cGCGCGUGAuucacauacaGCCgAGCAagcaGACAGUCg -3' miRNA: 3'- cCGCGCGCU----------UGG-UUGUag--CUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 22458 | 0.67 | 0.888544 |
Target: 5'- cGUGCGCGGcgaucgcacgcuGCCGGCGUCGAg----- -3' miRNA: 3'- cCGCGCGCU------------UGGUUGUAGCUguuaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 10046 | 0.67 | 0.888544 |
Target: 5'- cGCGCGCG--UCAGCGccUCGACGAg-- -3' miRNA: 3'- cCGCGCGCuuGGUUGU--AGCUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 43687 | 0.67 | 0.903049 |
Target: 5'- aGCGCGCGcGCCAG-AUCGAaCAcgCc -3' miRNA: 3'- cCGCGCGCuUGGUUgUAGCU-GUuaGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 47423 | 0.67 | 0.903049 |
Target: 5'- cGGUGCucgcaccaGuCGAGCCAcGCGUCGACGggCa -3' miRNA: 3'- -CCGCG--------C-GCUUGGU-UGUAGCUGUuaGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 16704 | 0.67 | 0.903049 |
Target: 5'- aGCGCGCGcACCGcCuUCG-CGGUCg -3' miRNA: 3'- cCGCGCGCuUGGUuGuAGCuGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 35661 | 0.67 | 0.903049 |
Target: 5'- -cCGCGCGAGauCCAGCG-CGACGAc-- -3' miRNA: 3'- ccGCGCGCUU--GGUUGUaGCUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 6077 | 0.67 | 0.903049 |
Target: 5'- cGUGaCGCGGGCCGAaGUCGuucuuuGCGAUCa -3' miRNA: 3'- cCGC-GCGCUUGGUUgUAGC------UGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 3301 | 0.68 | 0.829415 |
Target: 5'- aGGCGCaGCGcgucGAUCAgcgcuGCAUCGACGuUCg -3' miRNA: 3'- -CCGCG-CGC----UUGGU-----UGUAGCUGUuAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 42380 | 0.68 | 0.838597 |
Target: 5'- -uCGUGCGAAgCGGCGUCGA-GAUCa -3' miRNA: 3'- ccGCGCGCUUgGUUGUAGCUgUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 30265 | 0.68 | 0.85626 |
Target: 5'- cGGCGCcugguauuggaGCGAGCCAGUAUCGGgAAg-- -3' miRNA: 3'- -CCGCG-----------CGCUUGGUUGUAGCUgUUaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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