Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12216 | 5' | -50.7 | NC_003309.1 | + | 3301 | 0.68 | 0.829415 |
Target: 5'- aGGCGCaGCGcgucGAUCAgcgcuGCAUCGACGuUCg -3' miRNA: 3'- -CCGCG-CGC----UUGGU-----UGUAGCUGUuAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 4183 | 0.66 | 0.934266 |
Target: 5'- aGGCaGCGCGGaugucguccGCCAGC-UCGAacgcgcCGGUCg -3' miRNA: 3'- -CCG-CGCGCU---------UGGUUGuAGCU------GUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 4686 | 0.73 | 0.555678 |
Target: 5'- cGGcCGCGCGAGCCGaaacgcuacggcgcGCAuucgcgccUUGACGAUCg -3' miRNA: 3'- -CC-GCGCGCUUGGU--------------UGU--------AGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 5063 | 0.68 | 0.85626 |
Target: 5'- gGGCGCgGCG-GCCGgugccGCGaCGGCGGUCg -3' miRNA: 3'- -CCGCG-CGCuUGGU-----UGUaGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 5562 | 0.69 | 0.800593 |
Target: 5'- -uCGCGCGcGCCGAUcgCGGCGGUg- -3' miRNA: 3'- ccGCGCGCuUGGUUGuaGCUGUUAga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 6077 | 0.67 | 0.903049 |
Target: 5'- cGUGaCGCGGGCCGAaGUCGuucuuuGCGAUCa -3' miRNA: 3'- cCGC-GCGCUUGGUUgUAGC------UGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 6750 | 0.71 | 0.716407 |
Target: 5'- cGGCGCGCGcauacGGCCAACGUgcuUGACcggCUg -3' miRNA: 3'- -CCGCGCGC-----UUGGUUGUA---GCUGuuaGA- -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 9693 | 0.75 | 0.48311 |
Target: 5'- cGCGCGCGAugCGcagcACGUCGuucugcGCAAUCg -3' miRNA: 3'- cCGCGCGCUugGU----UGUAGC------UGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 10046 | 0.67 | 0.888544 |
Target: 5'- cGCGCGCG--UCAGCGccUCGACGAg-- -3' miRNA: 3'- cCGCGCGCuuGGUUGU--AGCUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 11828 | 0.67 | 0.87293 |
Target: 5'- cGCGCGCccGCCGcgGCuuucgaauccuuGUCGGCGAUCg -3' miRNA: 3'- cCGCGCGcuUGGU--UG------------UAGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 11986 | 0.7 | 0.748982 |
Target: 5'- cGCGCGCGGcaGCCGcggcgcgcgccGCGUCGGCGu--- -3' miRNA: 3'- cCGCGCGCU--UGGU-----------UGUAGCUGUuaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 12465 | 0.69 | 0.810402 |
Target: 5'- cGCGCGCGuuUCAccCAUCGACA-UCg -3' miRNA: 3'- cCGCGCGCuuGGUu-GUAGCUGUuAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 12863 | 0.73 | 0.559012 |
Target: 5'- cGCGCGCGccgaaAACCGcgcgAgGUCGGCGAUCa -3' miRNA: 3'- cCGCGCGC-----UUGGU----UgUAGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 16204 | 0.71 | 0.660513 |
Target: 5'- cGGCcgGCGCGAacucgcACCAGuCGUCGACGccgAUCg -3' miRNA: 3'- -CCG--CGCGCU------UGGUU-GUAGCUGU---UAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 16704 | 0.67 | 0.903049 |
Target: 5'- aGCGCGCGcACCGcCuUCG-CGGUCg -3' miRNA: 3'- cCGCGCGCuUGGUuGuAGCuGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 16948 | 0.76 | 0.402966 |
Target: 5'- cGGCuGCGCGaAACCgAACGUCG-CGAUCg -3' miRNA: 3'- -CCG-CGCGC-UUGG-UUGUAGCuGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 19958 | 0.69 | 0.780425 |
Target: 5'- uGCGCGCGAuCCG-CuUCGACGcgCg -3' miRNA: 3'- cCGCGCGCUuGGUuGuAGCUGUuaGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 21751 | 0.69 | 0.820013 |
Target: 5'- aGGCGUgcaGCGAGCuCGAUcugcucUCGACGAUCc -3' miRNA: 3'- -CCGCG---CGCUUG-GUUGu-----AGCUGUUAGa -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 22085 | 0.7 | 0.748982 |
Target: 5'- aGGCGCGCGGcCCGAUGUUguaGGCGAa-- -3' miRNA: 3'- -CCGCGCGCUuGGUUGUAG---CUGUUaga -5' |
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12216 | 5' | -50.7 | NC_003309.1 | + | 22423 | 0.75 | 0.471619 |
Target: 5'- cGGCGCGCGAGaucuugcCCAACGcuUCGGCAu--- -3' miRNA: 3'- -CCGCGCGCUU-------GGUUGU--AGCUGUuaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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