Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12322 | 3' | -60.4 | NC_003324.1 | + | 30453 | 0.67 | 0.3988 |
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Target: 5'- gGCgGCAUGGCcgaGGGCgacGCCAUCaAGGCu -3' miRNA: 3'- -CGaCGUACCG---CCCG---CGGUGGcUUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 6042 | 0.66 | 0.435186 |
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Target: 5'- cGCUGCAUagccauucGGCGGGUaguucGCUGCCuGAuagccagcAGCGa -3' miRNA: 3'- -CGACGUA--------CCGCCCG-----CGGUGG-CU--------UCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 27375 | 0.66 | 0.443635 |
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Target: 5'- cGCgaagGCAaggGGCGGgaaauccGCGCCACCaacgcgGAAGCc -3' miRNA: 3'- -CGa---CGUa--CCGCC-------CGCGGUGG------CUUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 12037 | 0.66 | 0.454085 |
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Target: 5'- uGCuUGCcgGGCaGGGCGCCGacucuucuCCGaAAGaCGu -3' miRNA: 3'- -CG-ACGuaCCG-CCCGCGGU--------GGC-UUC-GC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 40319 | 0.66 | 0.47342 |
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Target: 5'- gGCgGCAgucGaGCGGGaGCCagcACCGAAGCc -3' miRNA: 3'- -CGaCGUa--C-CGCCCgCGG---UGGCUUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 3642 | 1.09 | 0.000327 |
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Target: 5'- uGCUGCAUGGCGGGCGCCACCGAAGCGc -3' miRNA: 3'- -CGACGUACCGCCCGCGGUGGCUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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