Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12322 | 3' | -60.4 | NC_003324.1 | + | 14515 | 0.71 | 0.236032 |
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Target: 5'- cGCcgGCAUGGgcccagagcCGGGCGugauccCCAUCGAGGCGu -3' miRNA: 3'- -CGa-CGUACC---------GCCCGC------GGUGGCUUCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 32819 | 0.73 | 0.157543 |
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Target: 5'- gGC-GCAUGGCGGGCaGCCGCCucgaucaucgccacuGAcGCGc -3' miRNA: 3'- -CGaCGUACCGCCCG-CGGUGG---------------CUuCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 17834 | 0.73 | 0.168308 |
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Target: 5'- cCUG-AUGGCGGGUGUCACCGGcacaaucGGCu -3' miRNA: 3'- cGACgUACCGCCCGCGGUGGCU-------UCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 18103 | 0.74 | 0.143903 |
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Target: 5'- --cGCAUGGCccguGGCGCCGgccaaucgguauCCGAAGCGa -3' miRNA: 3'- cgaCGUACCGc---CCGCGGU------------GGCUUCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 16223 | 0.75 | 0.125798 |
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Target: 5'- -aUGCcUGGCGcGGCccccGCUACCGAAGCGc -3' miRNA: 3'- cgACGuACCGC-CCG----CGGUGGCUUCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 3642 | 1.09 | 0.000327 |
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Target: 5'- uGCUGCAUGGCGGGCGCCACCGAAGCGc -3' miRNA: 3'- -CGACGUACCGCCCGCGGUGGCUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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