Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12322 | 3' | -60.4 | NC_003324.1 | + | 30468 | 0.69 | 0.30935 |
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Target: 5'- cGUUGCG-GGuCGaaucGGCGCCGCCG-AGCa -3' miRNA: 3'- -CGACGUaCC-GC----CCGCGGUGGCuUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 32819 | 0.73 | 0.157543 |
|
Target: 5'- gGC-GCAUGGCGGGCaGCCGCCucgaucaucgccacuGAcGCGc -3' miRNA: 3'- -CGaCGUACCGCCCG-CGGUGG---------------CUuCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 38319 | 0.67 | 0.378778 |
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Target: 5'- uGCaGCAUGucuuUGGGCGUcucgaucacgagggCACCGggGCGg -3' miRNA: 3'- -CGaCGUACc---GCCCGCG--------------GUGGCuuCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 40319 | 0.66 | 0.47342 |
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Target: 5'- gGCgGCAgucGaGCGGGaGCCagcACCGAAGCc -3' miRNA: 3'- -CGaCGUa--C-CGCCCgCGG---UGGCUUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 48805 | 0.66 | 0.425909 |
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Target: 5'- --aGCc-GGaaGGUGCCACCGAGGCc -3' miRNA: 3'- cgaCGuaCCgcCCGCGGUGGCUUCGc -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 49967 | 0.69 | 0.287767 |
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Target: 5'- --aGCAUGGCGaucGGCGCUGCCcugaugGggGCa -3' miRNA: 3'- cgaCGUACCGC---CCGCGGUGG------CuuCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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