Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12322 | 3' | -60.4 | NC_003324.1 | + | 8274 | 0.68 | 0.36442 |
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Target: 5'- -gUGCcaGGCGcGCGCCGCCGGgcAGUGa -3' miRNA: 3'- cgACGuaCCGCcCGCGGUGGCU--UCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 6042 | 0.66 | 0.435186 |
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Target: 5'- cGCUGCAUagccauucGGCGGGUaguucGCUGCCuGAuagccagcAGCGa -3' miRNA: 3'- -CGACGUA--------CCGCCCG-----CGGUGG-CU--------UCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 5710 | 0.7 | 0.274047 |
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Target: 5'- -aUGCAccGCGGGCGCaACCGAaaccuccguuGGCGg -3' miRNA: 3'- cgACGUacCGCCCGCGgUGGCU----------UCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 5491 | 0.69 | 0.30935 |
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Target: 5'- cGCcGCAgGuGCGGGCGCCAucuuCCGAGcCGa -3' miRNA: 3'- -CGaCGUaC-CGCCCGCGGU----GGCUUcGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 3642 | 1.09 | 0.000327 |
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Target: 5'- uGCUGCAUGGCGGGCGCCACCGAAGCGc -3' miRNA: 3'- -CGACGUACCGCCCGCGGUGGCUUCGC- -5' |
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| 12322 | 3' | -60.4 | NC_003324.1 | + | 3629 | 0.69 | 0.316814 |
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Target: 5'- gGCUGCAuagcccuucuUGGCGGGgGCCucGuuGAGGg- -3' miRNA: 3'- -CGACGU----------ACCGCCCgCGG--UggCUUCgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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