Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12324 | 3' | -58.4 | NC_003324.1 | + | 4969 | 1.08 | 0.000558 |
Target: 5'- aAGCGAUAACGCCAGCCUGACGGCCCAu -3' miRNA: 3'- -UCGCUAUUGCGGUCGGACUGCCGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 5890 | 0.75 | 0.15634 |
Target: 5'- uGGgGAUGACgGCCugcGCCuUGAUGGCCCGa -3' miRNA: 3'- -UCgCUAUUG-CGGu--CGG-ACUGCCGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 7755 | 0.68 | 0.437966 |
Target: 5'- cGGCGGUGugcagcgacacauaaACGCCAGCCggcacUGcCGGCgCGa -3' miRNA: 3'- -UCGCUAU---------------UGCGGUCGG-----ACuGCCGgGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 8375 | 0.67 | 0.473083 |
Target: 5'- uGCgGAUGACGCUGGCUgcGACGGCg-- -3' miRNA: 3'- uCG-CUAUUGCGGUCGGa-CUGCCGggu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 9284 | 0.72 | 0.238511 |
Target: 5'- cGCGuGUAACGCCAGCa--GCGGUCCu -3' miRNA: 3'- uCGC-UAUUGCGGUCGgacUGCCGGGu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 11146 | 0.7 | 0.299025 |
Target: 5'- cGCuu---CGCCGGUCUGGCGGCCg- -3' miRNA: 3'- uCGcuauuGCGGUCGGACUGCCGGgu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 11619 | 0.66 | 0.503398 |
Target: 5'- cAGCGAUGaacguGCGCUugaacgacggcGGUCUGACGccgaucaacaaGCCCAu -3' miRNA: 3'- -UCGCUAU-----UGCGG-----------UCGGACUGC-----------CGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 14424 | 0.67 | 0.453393 |
Target: 5'- uGGgGAUcACGcCCGGCuCUGGCG-CCCAu -3' miRNA: 3'- -UCgCUAuUGC-GGUCG-GACUGCcGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 14608 | 0.75 | 0.160621 |
Target: 5'- aGGCGGUAGCGUggCGGCCggagcuACGGCCCu -3' miRNA: 3'- -UCGCUAUUGCG--GUCGGac----UGCCGGGu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 16248 | 0.67 | 0.457296 |
Target: 5'- uGCGGUGccCGCCucggugccagcgauGCCUGgcGCGGCCCc -3' miRNA: 3'- uCGCUAUu-GCGGu-------------CGGAC--UGCCGGGu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 17789 | 0.66 | 0.555652 |
Target: 5'- --gGGUAuCGCCAGCCUGAucuuCGGCg-- -3' miRNA: 3'- ucgCUAUuGCGGUCGGACU----GCCGggu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 19488 | 0.7 | 0.329515 |
Target: 5'- gGGCGAUAGCGUCGGCgUcGGCGuGCUUc -3' miRNA: 3'- -UCGCUAUUGCGGUCGgA-CUGC-CGGGu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 21195 | 0.67 | 0.493196 |
Target: 5'- uGCGcaAGCGCCAGCCcGACGuggaaGCCg- -3' miRNA: 3'- uCGCuaUUGCGGUCGGaCUGC-----CGGgu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 22037 | 0.67 | 0.463183 |
Target: 5'- aGGUGcAUcGCGCC-GCCgacgaGAUGGCCCGc -3' miRNA: 3'- -UCGC-UAuUGCGGuCGGa----CUGCCGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 25590 | 0.7 | 0.321682 |
Target: 5'- cGGCGGUGAUGacaUAGUCUGuCGGCCg- -3' miRNA: 3'- -UCGCUAUUGCg--GUCGGACuGCCGGgu -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 25794 | 0.66 | 0.510594 |
Target: 5'- uGGCGGUAcCGUCAuagucgagacgcacGCCUG-CGcGCCCGg -3' miRNA: 3'- -UCGCUAUuGCGGU--------------CGGACuGC-CGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 28829 | 0.69 | 0.348882 |
Target: 5'- uGCGcuUGACGCCAGCCUGuucgagcacgcgcuuGCGaGCCgGa -3' miRNA: 3'- uCGCu-AUUGCGGUCGGAC---------------UGC-CGGgU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 30264 | 0.71 | 0.277623 |
Target: 5'- cGGCGAUGAUGCgGGCCaagGcACGgucGCCCAa -3' miRNA: 3'- -UCGCUAUUGCGgUCGGa--C-UGC---CGGGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 31260 | 0.67 | 0.473083 |
Target: 5'- --gGAUAACcCgGGCCUGAUGGCgCAg -3' miRNA: 3'- ucgCUAUUGcGgUCGGACUGCCGgGU- -5' |
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12324 | 3' | -58.4 | NC_003324.1 | + | 31764 | 0.67 | 0.453393 |
Target: 5'- -cUGggGGCGCCAacGCCUGcCGGCCUc -3' miRNA: 3'- ucGCuaUUGCGGU--CGGACuGCCGGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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