Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12324 | 3' | -58.4 | NC_003324.1 | + | 34427 | 0.67 | 0.443716 |
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Target: 5'- -uCGGUGAUGUCGGCC-GACGGCaCAu -3' miRNA: 3'- ucGCUAUUGCGGUCGGaCUGCCGgGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 35030 | 0.66 | 0.545056 |
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Target: 5'- cAGCGGcGGCGgCAGCa--GCGGCCUg -3' miRNA: 3'- -UCGCUaUUGCgGUCGgacUGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 42191 | 0.68 | 0.434156 |
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Target: 5'- cGGCacu-GCGCCGGagUGAuCGGCCCAa -3' miRNA: 3'- -UCGcuauUGCGGUCggACU-GCCGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 42758 | 0.75 | 0.148087 |
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Target: 5'- cAGCGGU--UGCCGGuCCUGGUGGCCCGg -3' miRNA: 3'- -UCGCUAuuGCGGUC-GGACUGCCGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 43572 | 0.67 | 0.443716 |
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Target: 5'- cGCGc-AACGUCAGCCgauUGAgGGCCUg -3' miRNA: 3'- uCGCuaUUGCGGUCGG---ACUgCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 46989 | 0.67 | 0.483089 |
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Target: 5'- cGGCGAUAACGCCccucGGgCUGccuugaaccgcaGCGGCUa- -3' miRNA: 3'- -UCGCUAUUGCGG----UCgGAC------------UGCCGGgu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 48842 | 0.66 | 0.555652 |
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Target: 5'- gGGCGAccuugAGCGCUucgCUGAUGGCCUg -3' miRNA: 3'- -UCGCUa----UUGCGGucgGACUGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 49611 | 0.67 | 0.483089 |
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Target: 5'- uGGCGAUAcCGUCAuUCUGGCaGGCgCCAa -3' miRNA: 3'- -UCGCUAUuGCGGUcGGACUG-CCG-GGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 49704 | 0.67 | 0.443716 |
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Target: 5'- uGCGAUcguuGGCGCCuGCCagaaUGACGGUaucgCCAu -3' miRNA: 3'- uCGCUA----UUGCGGuCGG----ACUGCCG----GGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 56147 | 0.75 | 0.147685 |
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Target: 5'- cGCGGguuCGCCAGCCUggaagccGACGGCCg- -3' miRNA: 3'- uCGCUauuGCGGUCGGA-------CUGCCGGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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