Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12324 | 3' | -58.4 | NC_003324.1 | + | 7755 | 0.68 | 0.437966 |
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Target: 5'- cGGCGGUGugcagcgacacauaaACGCCAGCCggcacUGcCGGCgCGa -3' miRNA: 3'- -UCGCUAU---------------UGCGGUCGG-----ACuGCCGgGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 49704 | 0.67 | 0.443716 |
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Target: 5'- uGCGAUcguuGGCGCCuGCCagaaUGACGGUaucgCCAu -3' miRNA: 3'- uCGCUA----UUGCGGuCGG----ACUGCCG----GGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 16248 | 0.67 | 0.457296 |
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Target: 5'- uGCGGUGccCGCCucggugccagcgauGCCUGgcGCGGCCCc -3' miRNA: 3'- uCGCUAUu-GCGGu-------------CGGAC--UGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 22037 | 0.67 | 0.463183 |
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Target: 5'- aGGUGcAUcGCGCC-GCCgacgaGAUGGCCCGc -3' miRNA: 3'- -UCGC-UAuUGCGGuCGGa----CUGCCGGGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 31260 | 0.67 | 0.473083 |
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Target: 5'- --gGAUAACcCgGGCCUGAUGGCgCAg -3' miRNA: 3'- ucgCUAUUGcGgUCGGACUGCCGgGU- -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 8375 | 0.67 | 0.473083 |
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Target: 5'- uGCgGAUGACGCUGGCUgcGACGGCg-- -3' miRNA: 3'- uCG-CUAUUGCGGUCGGa-CUGCCGggu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 21195 | 0.67 | 0.493196 |
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Target: 5'- uGCGcaAGCGCCAGCCcGACGuggaaGCCg- -3' miRNA: 3'- uCGCuaUUGCGGUCGGaCUGC-----CGGgu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 48842 | 0.66 | 0.555652 |
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Target: 5'- gGGCGAccuugAGCGCUucgCUGAUGGCCUg -3' miRNA: 3'- -UCGCUa----UUGCGGucgGACUGCCGGGu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 17789 | 0.66 | 0.555652 |
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Target: 5'- --gGGUAuCGCCAGCCUGAucuuCGGCg-- -3' miRNA: 3'- ucgCUAUuGCGGUCGGACU----GCCGggu -5' |
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| 12324 | 3' | -58.4 | NC_003324.1 | + | 4969 | 1.08 | 0.000558 |
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Target: 5'- aAGCGAUAACGCCAGCCUGACGGCCCAu -3' miRNA: 3'- -UCGCUAUUGCGGUCGGACUGCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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